Nandita Nath

US Patent:

20120329678, Dec 27, 2012

Filed:

Jun 27, 2012

Appl. No.:

13/535167

Inventors:

Feng Chen - Castro Valley CA, US
Ze Peng - Moraga CA, US
Zhiying Zhao - Danville CA, US
Nandita Nath - Fremont CA, US
Jeff L. Froula - Walnut Creek CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C40B 50/06
C40B 40/06

US Classification:

506 16, 506 26

Abstract:

The present disclosure provides methods for generating mate-pair libraries using a recombinase/recombination site system. The method allows for increased insert size, improved efficiency and simplicity of the steps involved, and improved data generation. Mate-pair libraries are helpful in providing positional information for the assembly of sequence data from short read sequencing platforms. The disclosure also embodies the mate-pair libraries as generated from these methods.