Michel M Chartrain from 1213 Boulevard, Westfield, NJ 07090, age 68, Phone: 908 654-4459

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Position:

Distinguished Senior Investigator at Merck

Location:

Greater New York City Area

Industry:

Pharmaceuticals

Work:

Merck since Aug 1986
Distinguished Senior Investigator

Education:

University of Wisconsin-Madison 1982 - 1986
PH.D., Microbiology

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Position:

Distinguished Senior Investigator at Merck and Co

Location:

Greater New York City Area

Industry:

Biotechnology

Work:

Merck and Co
Distinguished Senior Investigator

Process For Large Scale Production Of Plasmid Dna By Fermentation
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US Patent:

7998732, Aug 16, 2011
Filed:

Jan 31, 2005
Appl. No.:

10/588295
Inventors:

Michel Chartrain - Westfield NJ, US
Laura Kizer Bentley - Monmouth NJ, US
Barbara Ann Krulewicz - Bloomfield NJ, US
Kristin M. Listner - East Windsor NJ, US
Wen-jun Sun - Edison NJ, US
Chanyong Brian Lee - Thousand Oaks CA, US
Assignee:

Merck Sharp & Dohme Corp. - Rahway NJ
International Classification:

C12N 15/63
C12N 1/21
C12N 15/70
US Classification:

4353201, 4352523, 435471
Abstract:

The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of , including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.

Process For Large Scale Production Of Plasmid Dna By Fermentation
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US Patent:

8603798, Dec 10, 2013
Filed:

Jul 25, 2011
Appl. No.:

13/190137
Inventors:

Michel Chartrain - Westfield NJ, US
Laura Kizer Bentley - Monmouth NJ, US
Barbara Ann Krulewicz - Bloomfield NJ, US
Kristin M. Listner - East Windsor NJ, US
Wen-jun Sun - Edison NJ, US
Chanyong Brian Lee - Thousand Oaks CA, US
Assignee:

Merck Sharp & Dohme Corp. - Rahway NJ
International Classification:

C12N 1/20
C12N 15/63
US Classification:

43525233, 4353201
Abstract:

The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of , including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.

Asymmetric Bioreduction Of 6-Bromo-2-Tetralone To (S)-6-Bromo-2 Tetraol By Trichosporon Capitatum
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US Patent:

54647622, Nov 7, 1995
Filed:

Jun 24, 1994
Appl. No.:

8/265176
Inventors:

Michel M. Chartrain - Westfield NJ
Jayanthi Reddy - Scotch Plains NJ
David M. Tschaen - Holmdel NJ
Assignee:

Merck & Co., Inc. - Rahway NJ
International Classification:

C12P 722
C12P 4100
US Classification:

435156
Abstract:

Stereospecific (S)-6-bromo-2-tetraol is a key intermediate in the chemical synthesis of the chiral drug candidate, MK499, a ventricular arrythmias suppressant. The yeast strain Trichosporon capitatum (MY 1890) was employed for the bioconversion of 6-bromo-2-tetralone to the corresponding alcohol.

Microbial Method
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US Patent:

54910773, Feb 13, 1996
Filed:

Jul 20, 1994
Appl. No.:

8/277728
Inventors:

Michel M. Chartrain - Westfield NJ
George M. Garrity - Westfield NJ
Brian Heimbuch - North Brunswick NJ
Christopher Roberge - Clark NJ
Ali Shafiee - Westfield NJ
Assignee:

Merck & Co., Inc. - Rahway NJ
International Classification:

C12P 1716
C12P 1712
US Classification:

435118
Abstract:

The present invention provides a process for the stereoselective reduction of phenylalkyl ketones to their corresponding (S)-hydroxy compounds. The process utilizes the novel microorganism Microbacterium MB 5614 to effectuate the chiral reduction. The present invention also provides said novel Microbacterium, which has been deposited with the ATCC and assigned the accession number ATCC 55557.

Quantitative Conversion Of Indene To (1S,2R) Indene Oxide And (1S,2R)-Indandiol By Combination Of Haloperoxidase Bioconversion And Chemical Steps
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US Patent:

56058195, Feb 25, 1997
Filed:

May 19, 1995
Appl. No.:

8/445154
Inventors:

Michel M. Chartrain - Westfield NJ
Neal C. Connors - Fanwood NJ
George M. Garrity - Westfield NJ
Roger C. Olewinski - Milltown NJ
Thomas R. Verhoeven - Cranford NJ
Jinyou Zhang - Edison NJ
Assignee:

Merck & Co., Inc. - Rahway NJ
International Classification:

C12P 1702
C12P 722
C12P 102
C12P 500
US Classification:

435123
Abstract:

A process is disclosed that quantitatively bioconverts indene to (1S,2R)-indene oxide and (1S,2R)-indandiol, by the action of fungal haloperoxidase followed by various chemical step(s), e. g. , adjusting the pH.

Stereospecific Bioconversion Of Benzyl Acetoacetate To Benzyl-(S)-(+)-Hydroxybutyrate
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US Patent:

59587431, Sep 28, 1999
Filed:

Feb 28, 1997
Appl. No.:

8/803852
Inventors:

Michel Chartrain - Westfield NJ
James M. McNamara - Rahway NJ
Assignee:

Merck & Co., Inc. - Rahway NJ
International Classification:

C12P 700
C12P 762
C12P 4100
US Classification:

435135
Abstract:

A bioconversion process where benzyl acetoacetate is converted to benzyl-(S)-(+)-3-hydroxybutyrate.

Enantioselective Bioreduction Using Yeast
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US Patent:

61210269, Sep 19, 2000
Filed:

Jul 23, 1999
Appl. No.:

9/360202
Inventors:

Michel M. Chartrain - Westfield NJ
Barbara A. Krulewicz - Bloomfield NJ
Paul N. Devine - Lincroft NJ
David M. Tschaen - Holmdel NJ
Assignee:

Merck & Co., Inc. - Rahway NJ
International Classification:

C12P 700
C07D31744
US Classification:

435132
Abstract:

The invention relates to a class of enantiomerically pure intermediates represented by Formula I, where X is F, Cl, Br, or I, and a novel enantioselective bioreductive process using yeast to form these intermediates, which are useful in the synthesis of endothelin antagonists, and the like. ##STR1##.

Resolution Of Racemic Indene Oxide To Yield (1S,2R)-Indene Oxide Using Diplodia Gossipina
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US Patent:

58495681, Dec 15, 1998
Filed:

Feb 20, 1996
Appl. No.:

8/603571
Inventors:

Michel M. Chartrain - Westfield NJ
Chris H. Senanayake - North Brunswick NJ
Jinyou Zhang - Edison NJ
Assignee:

Merck & Co., Inc. - Rahway NJ
International Classification:

C12P 1702
C12P 4100
US Classification:

435280
Abstract:

A process is disclosed that hydrolyzes, by the action of an epoxide hydrolase of Diplodia gossipina (alternatively named Diplodia gossypina) ATCC 16391 or ATCC 10936, the undesired enantiomer of racemic indene oxide, an epoxide of indan.