This invention relates to a biocatalytic process to produce terephthalic acid and isophthalic acid from p-xylene and m-xylene, respectively. Terephthalic acid has been prepared by oxidizing p-xylene with bacteria belonging to the genus Burkholderia. Conversion of p-xylene into terephthalic acid is accomplished by a single bacterial strain that produces all of the requisite enzymes. In addition, this invention relates to the preparation of isophthalic acid from a mixture of m- and p-xylene.
Bacterial Plasmid Having Genes Encoding Enzymes For The Degradation Of Aromatic Compounds
A bacterial plasmid has been isolated from Pseudomonas CT14 comprising genes encoding enzymes implicated in aromatic ring cleavage. Additionally, the novel genes encoding proteins involved in mercury tolerance and plasmid stability and replication have been identified. The strain from which the plasmid was isolated is useful in a variety of methods including methods for the degradation of aromatic compounds, particularly catechols.
3-Hydroxycarboxylic Acid Production And Use In Branched Polymers
Michael G. Bramucci - Folsom PA Robert Dicosimo - Rockland DE Robert Fallon - Elkton MD John E. Gavagan - Wilmington DE Frank Herkes - Wilmington DE Lech Wilczek - Wilmington DE
Assignee:
E. I. du Pont de Nemours and Company - Wilmington DE
International Classification:
C12P 742
US Classification:
435146, 435170, 435227, 435232
Abstract:
This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for -caprolactone in the preparation of highly branched copolyester.
Nucleic Acid Fragments For The Identification Of Bacteria In Industrial Wastewater Bioreactors
Unique bacterial strains and identifying 16S rDNA sequences have been isolated from activated wastewater sludge. The 16S rDNA sequences are diagnostic for organisms which are key to the health of activated sludge wastewater systems.
A plasmid has been isolated from strain AN12 comprising a unique replication protein. The replication protein may be used in a variety of cloning and expression vectors and particularly in shuttle vectors for the expression of heterologous genes in sp.
Michael G. Bramucci - Folsom PA, US Patricia C. Brzostowicz - West Chester PA, US Qiong Cheng - Wilmington DE, US Kristy N Kostichka - Wilmington DE, US Pierre E. Rouviere - Wilmington DE, US Vasantha Nagarajan - Wilmington DE, US Luan Tao - Claymont DE, US Stuart M. Thomas - Wilmington DE, US
Assignee:
E.I. du Pont de Nemours and Company - Wilmington DE
Genes have been isolated from AN12 strain encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from a strain. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.
Temperature Sensitive Mutant Derivatives Of The Broad Host Range Plasmid Pbhr1
E. I. du Pont de Nemours and Company - Wilmington DE
International Classification:
C12N 1/21 C12N 15/63 C12N 15/11
US Classification:
43525233, 43525234, 4352523, 4353201, 536 231
Abstract:
The present invention describes a mutant plasmid replication control region having the ability to convey temperature sensitivity to the plasmid on which it resides. The mutant replication control region is based on a similar region isolated from pBRH1. Plasmids containing this rggeplication control region cannot be classed as belonging to any known incompatibility group and thus may co-exist with a broad range of other plasmids in a single host.
Nucleic Acid Fragments For The Identification Of Bacteria In Industrial Wastewater Bioreactors
Unique bacterial strains and identifying 16S rDNA sequences have been isolated from activated wastewater sludge. The 16S rDNA sequences are diagnostic for organisms which are key to the health of activated sludge wastewater systems.