Max J Costa

Author:

Max Costa

ISBN #:

0896030172

US Patent:

20020182621, Dec 5, 2002

Filed:

Jan 25, 2002

Appl. No.:

10/057832

Inventors:

Max Costa - Ramsey NJ, US
Konstantin Salnikow - Waldwick NJ, US
Herman Yee - Pomona NY, US

International Classification:

C12Q001/68
G01N033/53
G01N033/567

US Classification:

435/006000, 435/007200

Abstract:

The present invention relates to a gene, known as CAP43, and its gene product. In particular, the application demonstrates that the CAP43 gene and its gene product are expressed at elevated in certain diseased cells and tissues, such as cancer cells and tissues. The invention therefore provides novel uses for the CAP43 gene and gene product, including novel diagnostic methods for diagnosing diseases, such as cancer, by detected elevated levels of CAP43 expression. Methods are also provided for identifying diseased cells and tissue, particularly cancer cells and tissue, by identifying cells and tissues that express elevated levels of CAP43. Moreover, the invention also provides novel therapeutic methods for treating diseases, such as cancer, by targeting therapeutic compounds to cells expressing CAP43. Pharmaceutical compositions which may be used in such methods of treatment are also provided.

US Patent:

55455298, Aug 13, 1996

Filed:

Mar 27, 1995

Appl. No.:

8/410389

Inventors:

Max Costa - Ramsey NJ
Anatoly V. Zhitkovich - Greenwood NY

Assignee:

New York University - New York NY

International Classification:

C12Q 148
C12Q 168
C12Q 1533

US Classification:

435 6

Abstract:

Since sodium dodecyl sulfate (SDS) binds tightly to proteins but not to DNA, the addition of potassium chloride (KCl) to SDS lysates of cells, nuclei or mitochondria results in the formation of an insoluble precipitate which includes all proteins and detergent-resistant DNA-protein complexes separated from free DNA in the supernatant. The amount of SDS-precipitable DNA represents a measure of crosslinked DNA-protein. This precipitation method is useful for determining or quantitating crosslinked DNA-protein formed in cells, nuclei or mitochondria of animals following their exposure to crosslinking agents such as chromate, nickel, cis-Pt (II) diammine dichloride and formaldehyde. The method is also useful for evaluating agents for their ability to induce covalent DNA-protein crosslinking.