Markus G Manz

US Patent:

7300760, Nov 27, 2007

Filed:

Dec 15, 2003

Appl. No.:

10/737576

Inventors:

Irving L. Weissman - Redwood City CA, US
David Jeffrey Traver - West Roxbury MA, US
Koichi Akashi - Boston MA, US
Markus Gabriel Manz - Palo Alto CA, US
Toshihiro Miyamoto - Menlo Park CA, US

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Palo Alto CA

International Classification:

G01N 33/53
C12N 5/00
C12N 5/02

US Classification:

435 71, 435325, 435372, 435373, 435374

Abstract:

A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for myeloid lineages, but lacking the potential to differentiate into lymphoid lineages. This population is further divided into specific myeloid progenitor subsets, including a common myeloid progenitor cells (CMP), megakaryocyte/erythroid progenitor cells (MEP) and granulocyte/monocyte lineage progenitor (GMP). Methods are provided for the isolation and culture of these subpopulations. The CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid/megakaryocytic or myelomonocytic lineages. The cell enrichment methods employ reagents that specifically recognize Thy-1; and IL-7Rα, in conjunction with other markers expressed on lineage committed cells. These cells give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo.

US Patent:

7618654, Nov 17, 2009

Filed:

Oct 18, 2007

Appl. No.:

11/874841

Inventors:

Irving L. Weissman - Redwood City CA, US
David Jeffrey Traver - West Roxbury MA, US
Koichi Akashi - Palo Alto CA, US
Markus Gabriel Manz - Palo Alto CA, US
Toshihiro Miyamoto - Menlo Park CA, US

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Palo Alto CA

International Classification:

A61K 35/28
G01N 33/53

US Classification:

424577, 435 71

Abstract:

A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for myeloid lineages, but lacking the potential to differentiate into lymphoid lineages. This population is further divided into specific myeloid progenitor subsets, including a common myeloid progenitor cells (CMP), megakaryocyte/erythroid progenitor cells (MEP) and granulocyte/monocyte lineage progenitor (GMP). Methods are provided for the isolation and culture of these subpopulations. The CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid/megakaryocytic or myelomonocytic lineages. Tηε χελλ ενιχημεντ μετηoδσ εμπλoψεαγεντσ τηατ σπεχαλλψεψoγνιζε Tηψ-1; ανδIΛ-7 Pα, in conjunction with other markers expressed on lineage committed cells. These cells give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo.

US Patent:

20020086422, Jul 4, 2002

Filed:

Sep 17, 2001

Appl. No.:

09/956279

Inventors:

Irving Weissman - Redwood City CA, US
David Traver - West Roxbury MA, US
Koichi Akashi - Palo Alto CA, US
Markus Manz - Palo Alto CA, US
Toshihiro Miyamoto - Menlo Park CA, US

International Classification:

C12N005/08
C12N005/06

US Classification:

435/368000, 435/354000, 435/372000

Abstract:

A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for myeloid lineages, but lacking the potential to differentiate into lymphoid lineages. This population is further divided into specific myeloid progenitor subsets, including a common myeloid progenitor cells (CMP), megakaryocyte/erythroid progenitor cells (MEP) and granulocyte/monocyte lineage progenitor (GMP). Methods are provided for the isolation and culture of these subpopulations. The CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid/megakaryocytic or myelomonocytic lineages. The cell enrichment methods employ reagents that specifically recognize Thy-1; and IL-7R, in conjunction with other markers expressed on lineage committed cells. These cells give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo.