Mark J Pykett

US Patent:

6548299, Apr 15, 2003

Filed:

May 18, 2000

Appl. No.:

09/574749

Inventors:

Mark J. Pykett - Newton MA 02165
Michael Rosenzweig - Boston MA 02116
David T. Scadden - Weston MA 02498
Mark C. Poznansky - Charlestown MA 02129

International Classification:

C12N 506

US Classification:

435377, 435363, 435372, 4353723, 435373, 435395, 435402, 435352, 435 355, 623 16

Abstract:

The invention relates to a method for lymphoid tissue-specific cell production from hematopoietic progenitor cells in unique, three-dimensional culture devices, in the presence of lymphoreticular stromal cells and in the absence of exogenously added growth factors. The resulting differentiated progeny. The lymphoid tissue-specific cells may be isolated at any sequential stage of differentiation and further expanded. The lymphoid tissue-specific cells also may be genetically altered at any stage of the process.

US Patent:

6645489, Nov 11, 2003

Filed:

May 10, 2002

Appl. No.:

10/143540

Inventors:

Mark J. Pykett - Boxford MA
Michael Rosenzweig - Boston MA
Richard B. Kaplan - Beverly Hills CA

Assignee:

Cytomatrix, LLC - Woburn MA

International Classification:

C12N 508

US Classification:

424 937, 435372, 435402, 4352891

Abstract:

The invention pertains to methods and devices for the long term, in vitroculture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.

US Patent:

7067316, Jun 27, 2006

Filed:

Nov 10, 2003

Appl. No.:

10/705720

Inventors:

Mark J. Pykett - Boxford MA, US
Michael Rosenzweig - Boston MA, US
Richard B. Kaplan - Beverly Hills CA, US

Assignee:

Cytomatrix, LLC - Woburn MA

International Classification:

C12N 5/02

US Classification:

435402, 435372

Abstract:

The invention pertains to methods and devices for the long term, in vitroculture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.

US Patent:

7192769, Mar 20, 2007

Filed:

May 31, 2002

Appl. No.:

10/161097

Inventors:

Mark J. Pykett - Boxford MA, US
Michael Rosenzweig - Boston MA, US
David T. Scadden - Weston MA, US
Mark C. Poznansky - Charlestown MA, US

Assignee:

Cytomatrix, LLC - Chelmsford MA

International Classification:

C12N 5/06

US Classification:

435373, 435377, 435382, 435395, 435399, 4353723

Abstract:

The invention relates to a method for lymphoid tissue-specific cell production from hematopoietic progenitor cells in unique, three-dimensional culture devices, in the presence of antigen presenting cells and lymphoreticular stromal cells, and in the absence of exogenously added growth factors. The resulting lymphoid tissue-specific cells may be isolated at any sequential stage of differentiation and further expanded. The lymphoid tissue-specific cells also may be genetically altered at any stage of the process.

US Patent:

20060084170, Apr 20, 2006

Filed:

May 23, 2003

Appl. No.:

10/515075

Inventors:

Mark Pykett - Boxford MA, US
Michael Rosenzweig - Boston MA, US
Todd Upton - Chestnut Hill MA, US

International Classification:

C12N 5/08

US Classification:

435372000

Abstract:

The invention pertains to methods and devices for the in vitro culture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors. The hematopoietic progenitor cells are cultured in the absence of exogenously added hematopoietic growth factors without loss in cell progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency.

US Patent:

20070148769, Jun 28, 2007

Filed:

Jun 26, 2006

Appl. No.:

11/474931

Inventors:

Mark Pykett - Boxford MA, US
Michael Rosenzweig - Boston MA, US
Richard Kaplan - Beverly Hills CA, US

Assignee:

Cytomatrix, LLC - Woburn MA

International Classification:

C12N 5/08
C12N 5/06
C12M 3/00

US Classification:

435325000, 435372000, 435289100

Abstract:

The invention pertains to methods and devices for the long term, in vitroculture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.

US Patent:

20080063652, Mar 13, 2008

Filed:

Mar 29, 2005

Appl. No.:

10/594806

Inventors:

Mark J. Pykett - Boxford MA, US
Michael Rosenzweig - Boston MA, US

Assignee:

Cytomatrix, LLC - Chelmsford MA

International Classification:

A61K 35/00
A61K 39/00
A61P 43/00
C12N 5/08

US Classification:

4241841, 424 9371, 435375

Abstract:

The invention provides culture systems for the generation, expansion and isolation of regulatory T cells. These culture systems can be used to screen for factors that modulate regulatory T cell development. Regulatory T cells derived from such cultures can be used in various in vitro and in vivo applications, as can culture populations depleted of such cells.

US Patent:

20080064101, Mar 13, 2008

Filed:

Jan 31, 2007

Appl. No.:

11/701172

Inventors:

Mark Pykett - Boxford MA, US
Michael Rosenzweig - Boston MA, US
David Scadden - Weston MA, US
Mark Poznansky - Charlestown MA, US

Assignee:

Cytomatrix, LLC - Chelmsford MA
The General Hospital Corporation - Boston MA

International Classification:

C12N 5/06

US Classification:

435373000

Abstract:

The invention relates to a method for lymphoid tissue-specific cell production from hematopoietic progenitor cells in unique, three-dimensional culture devices, in the presence of antigen presenting cells and lymphoreticular stromal cells, and in the absence of exogenously added growth factors. The resulting lymphoid tissue-specific cells may be isolated at any sequential stage of differentiation and further expanded. The lymphoid tissue-specific cells also may be genetically altered at any stage of the process.