Marilyn Menger

US Patent:

6447995, Sep 10, 2002

Filed:

Oct 4, 2000

Appl. No.:

09/679439

Inventors:

Marilyn Menger - Derwood MD

Assignee:

GenVec, Inc. - Gaithersburg MD

International Classification:

C12Q 100

US Classification:

435 5, 435 8, 4353201, 4242331

Abstract:

The present invention provides methods of detecting and/or characterizing the viral vector particle content of a medium. A medium is provided and contacted with an excitation energy such that, if a viral vector particle is in the medium, an electron associated with the intrinsically fluorogenic portion of the viral vector particle will be raised to an excited energy state. The excited electron is permitted to emit radiation having an emission wavelength which is detected. The viral vector particle content of the medium then can be evaluated by comparing the detected emission wavelength with a standard signal. For example, the number of viral vector particles in a medium can be quantified by comparing the detected wavelength and its corresponding intensity to a standard signal. Similar methods for evaluating the adenoviral vector particle content of a medium and the intrinsically fluorogenic adenoviral structural protein content of a medium are provided.

US Patent:

6586226, Jul 1, 2003

Filed:

Nov 30, 2001

Appl. No.:

09/997909

Inventors:

Marilyn Menger - Germantown MD
Imre Kovesdi - Rockville MD

Assignee:

GenVec, Inc. - Gaithersburg MD

International Classification:

C12N 702

US Classification:

435239, 530414

Abstract:

A method of enriching a solution of an adenovirus comprising applying a mixed solution comprising an adenovirus and at least one undesired type of biomolecule to an anion exchange chromatography resin containing a binding moiety selected from the group consisting of dimethylaminopropyl, dimethylaminobutyl, dimethylaminoisobutyl, and dimethylaminopentyl and eluting the adenovirus from the chromatography resin. Also provided is a method of purifying an adenovirus from adenovirus-infected cells comprising lysing such cells, applying the lysate to a single chromatography resin, eluting the adenovirus from the chromatography resin, and collecting a fraction containing adenovirus that is substantially as pure as triple CsCl density gradient-purified adenovirus. The present method further provides a method of accurately quantifying the number of adenoviral particles in a solution of adenovirus comprising applying to and eluting from an anion exchange chromatography resin a sample solution of adenovirus, comparing the absorbance of the sample solution of adenovirus and the absorbance of a standard solution of adenovirus, and quantifying the number of adenoviral particles in the sample solution.

US Patent:

6630299, Oct 7, 2003

Filed:

Jun 25, 2001

Appl. No.:

09/888681

Inventors:

Marilyn Menger - Derwood MD

Assignee:

GenVec, Inc. - Gaithersburg MD

International Classification:

C12Q 100

US Classification:

435 5, 435 8, 4353201, 4351731, 4351739, 4242331

Abstract:

The present invention provides methods of detecting and/or characterizing the viral vector particle content of a medium. A medium is provided and contacted with an excitation energy such that, if a viral vector particle is in the medium, an electron associated with the intrinsically fluorogenic portion of the viral vector particle will be raised to an excited energy state. The excited electron is permitted to emit radiation having an emission wavelength which is detected. The viral vector particle content of the medium then can be evaluated by comparing the detected emission wavelength with a standard signal. For example, the number of viral vector particles in a medium can be quantified by comparing the detected wavelength and its corresponding intensity to a standard signal. Similar methods for evaluating the adenoviral vector particle content of a medium and the intrinsically fluorogenic adenoviral structural protein content of a medium are provided.

US Patent:

7141406, Nov 28, 2006

Filed:

May 5, 2003

Appl. No.:

10/429303

Inventors:

Miguel E Carrion - New Market MD, US
Marilyn Menger - Germantown MD, US
Imre Kovesdi - Rockville MD, US

Assignee:

GenVec, Inc. - Gaithersburg MD

International Classification:

C12N 7/02

US Classification:

435239

Abstract:

A method of enriching a solution for an adenovirus comprising contacting a solution containing an adenovirus with an anion exchange chromatography resin comprising an acrylate or sulphonamide linker such that the adenovirus binds to the chromatography resin and eluting the adenovirus from the resin with an eluant to obtain an enriched solution of adenovirus.

US Patent:

6383795, May 7, 2002

Filed:

Apr 22, 1999

Appl. No.:

09/296962

Inventors:

Marilyn Menger - Derwood MD
Imre Kovesdi - Rockville MD

Assignee:

GenVec, Inc. - Gaithersburg MD

International Classification:

C12N 702

US Classification:

435239, 435 5

Abstract:

A method of enriching a solution of an adenovirus comprising applying a mixed solution comprising an adenovirus and at least one undesired type of biomolecule to an anion exchange chromatography resin containing a binding moiety selected from the group consisting of dimethylaminopropyl, dimethylaminobutyl, dimethylaminoisobutyl, and dimethylaminopentyl and eluting the adenovirus from the chromatography resin. Also provided is a method of purifying an adenovirus from adenovirus-infected cells comprising lysing such cells, applying the lysate to a single chromatography resin, eluting the adenovirus from the chromatography resin, and collecting a fraction containing adenovirus that is substantially as pure as triple CsCl density gradient-purified adenovirus. The present method further provides a method of accurately quantifying the number of adenoviral particles in a solution of adenovirus comprising applying to and eluting from an anion exchange chromatography resin a sample solution of adenovirus, comparing the absorbance of the sample solution of adenovirus and the absorbance of a standard solution of adenovirus, and quantifying the number of adenoviral particles in the sample solution.