Marie Ann Johnson

US Patent:

6808904, Oct 26, 2004

Filed:

Dec 5, 2000

Appl. No.:

09/730525

Inventors:

Eric R. Ward - Durham NC
Sandra L. Volrath - Durham NC
Marie A. Johnson - Wendell NC
Sharon L. Potter - Raleigh NC

Assignee:

Syngenta Participations AG - Basel

International Classification:

C12P 1934

US Classification:

435 911, 435 912

Abstract:

The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an herbicide tolerant form of a plant protox enzyme. The present invention further provides shuffled DNA molecules encoding protox enzymes having enhanced tolerance to a herbicide that inhibits the protox activity encoded by a template DNA molecule from which the shuffled DNA molecule is derived.

US Patent:

20050081259, Apr 14, 2005

Filed:

Jul 23, 2003

Appl. No.:

10/625648

Inventors:

Peter Heifetz - Durham NC, US
Sandra Volrath - Durham NC, US
Marie Johnson - Wendell NC, US
Eric Ward - Durham NC, US

International Classification:

C07H021/04
A01H001/00
C12N015/82

US Classification:

800278000, 536023600, 435468000

Abstract:

The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an herbicide tolerant form of a plant protox enzyme. The present invention further provides shuffled DNA molecules encoding protox enzymes having enhanced tolerance to a herbicide that inhibits the protox activity encoded by a template DNA molecule from which the shuffled DNA molecule is derived.

US Patent:

63084589, Oct 30, 2001

Filed:

Feb 3, 2000

Appl. No.:

9/497698

Inventors:

Sandra L. Volrath - Durham NC
Marie A. Johnson - Wendell NC
Eric R. Ward - Durham NC
Peter B. Heifetz - Durham NC

Assignee:

Novartis Finance Corporation - New York NY

International Classification:

A01G 706
A01H 500

US Classification:

47 581

Abstract:

The present invention relates to methods for controlling the growth of undesired vegetation comprising applying an effective amount of a protox-inhibiting herbicide to a population of transgenic plants or plant seed transformed with a DNA sequence coding for a modified protox enzyme that is tolerant to a protox-inhibiting herbicide or to the locus where a population of the transgenic plants or plant seeds is cultivated.

US Patent:

60181052, Jan 25, 2000

Filed:

Feb 28, 1997

Appl. No.:

8/808323

Inventors:

Marie A. Johnson - Raleigh NC
Sandra L. Volrath - Durham NC
Eric R. Ward - Durham NC

Assignee:

Novartis Finance Corporation - New York NY

International Classification:

A01H 500
C07H 2104
C12N 514
C12N 1582

US Classification:

800298

Abstract:

Promoters naturally associated with plant protoporphyrinogen oxidase (protox) coding sequences, and derivatives thereof, are provided. These promoters can be used to control the expression of an operably linked heterologous coding sequence in a plant cell. These promoters are particularly useful for expressing modified forms of herbicide target enzymes, particularly modified forms of protox, to achieve tolerance to herbicides that inhibit the corresponding unmodified enzymes. Recombinant DNA molecules and chimeric genes comprising these promoters are provided, as well as plant tissue and plants containing such chimeric genes.

US Patent:

59396024, Aug 17, 1999

Filed:

Feb 28, 1997

Appl. No.:

8/808931

Inventors:

Sandra L. Volrath - Durham NC
Marie A. Johnson - Raleigh NC
Eric R. Ward - Durham NC
Peter B. Heifetz - Durham NC

Assignee:

Novartis Finance Corporation - New York NY

International Classification:

A01H 500
C12N 514
C12N 1529
C12N 1552
C12N 1582

US Classification:

800300

Abstract:

The present invention provides novel DNA sequences coding for plant protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, rape, rice and sorghum. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an inhibitor-resistant from of a plant protox enzyme.

US Patent:

60841559, Jul 4, 2000

Filed:

Jun 22, 1998

Appl. No.:

9/102420

Inventors:

Sandra L. Volrath - Durham NC
Marie A. Johnson - Raleigh NC
Eric R. Ward - Durham NC
Peter B. Heifetz - Durham NC

Assignee:

Novartis AG - Basel

International Classification:

A01H 500
A01H 510
C12N 514
C12N 1529
C12N 1552
C12N 1582

US Classification:

800300

Abstract:

The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an herbicide tolerant form of a plant protox enzyme.

US Patent:

60230126, Feb 8, 2000

Filed:

Mar 30, 1998

Appl. No.:

9/050603

Inventors:

Sandra L. Volrath - Durham NC
Marie A. Johnson - Raleigh NC
Sharon L. Potter - Raleigh NC
Eric R. Ward - Durham NC
Peter B. Heifetz - Durham NC

Assignee:

Novartis Finance Corporation - New York NY

International Classification:

C12N 1529
C12N 1551
C12N 1582

US Classification:

800300

Abstract:

The present invention provides novel DNA sequences isolated from soybean, wheat, cotton, sugar beet, rape, rice, sorghum, and sugar cane that coding for enzymes having protoporphyrinogen oxidase (protox) activity. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an inhibitor-resistant from of a plant protox enzyme.