Marian J Peris

US Patent:

20100311144, Dec 9, 2010

Filed:

May 28, 2010

Appl. No.:

12/790768

Inventors:

Marian PERIS - Belmont CA, US
Michael Phelan - Hayward CA, US
Barnett B. Rosenblum - San Jose CA, US
Stephen P. Hendricks - Los Gatos CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12N 9/10
C07H 21/04
C12N 15/63
C12N 1/00
C12N 7/01
C07H 21/02

US Classification:

435193, 536 232, 4353201, 435243, 4352351

Abstract:

Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

US Patent:

20100330570, Dec 30, 2010

Filed:

May 28, 2010

Appl. No.:

12/790760

Inventors:

Peter B. VANDER HORN - Encinitas CA, US
Cheng-Yao Chen - Carlsbad CA, US
Guobin Luo - Oceanside CA, US
Michael Previte - Carlsbad CA, US
Jamshid Temirov - Germantown TN, US
Theo Nikiforov - Carlsbad CA, US
Zhaohui Zhou - San Ramon CA, US
Hongye Sun - Belmont CA, US
Yufang Wang - Foster City CA, US
Stefanie Yukiko Nishimura - Mountain View CA, US
Hongyi Wang - Pearland TX, US
Marian Peris - Belmont CA, US
Barnett B. Rosenblum - San Jose CA, US
Michael Phelan - Hayward CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68
C12N 9/96

US Classification:

435 6, 435188

Abstract:

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

US Patent:

20110014612, Jan 20, 2011

Filed:

Mar 26, 2010

Appl. No.:

12/748359

Inventors:

STEPHEN P. HENDRICKS - LOS GATOS CA, US
MICHAEL PHELAN - HAYWARD CA, US
MARIAN PERIS - BELMONT CA, US
CHENG-YAO CHEN - CARLSBAD CA, US
DANIEL MAZUR - SAN DIEGO CA, US
XINZHAN PENG - CARLSBAD CA, US
AMY CASTILLO - HOUSTON TX, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - CARLSBAD CA

International Classification:

C12Q 1/68
C12P 19/34
C12N 9/12
C12N 5/10
C12N 15/63
C07H 21/04

US Classification:

435 6, 435 911, 435194, 435325, 4353201, 536 232

Abstract:

Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

US Patent:

20110159551, Jun 30, 2011

Filed:

Oct 8, 2010

Appl. No.:

12/900794

Inventors:

Roland Nagel - Santa Cruz CA, US
Stephen Hendricks - Los Gatos CA, US
Jennifer Berkman - San Francisco CA, US
Marian Peris - Belmont CA, US
Yulei Wang - Foster City CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 19/34
C12N 9/10

US Classification:

435 915, 435193

Abstract:

The present invention provides compositions and methods for a reverse transcription reaction using a reversibly inactivated reverse transcriptase enzyme. The reversibly inactivated reverse transcriptase enzyme results from a chemical modification which inactivates the reverse transcriptase enzyme. The activity of the reverse transcriptase enzyme is recovered by an incubation of the reaction mixture at elevated temperature prior to, or as part of the reverse transcription reaction. The reverse transcriptase enzyme of the present invention provides for a signficant reduction in non-specific reverse transcription from template nucleic acid molecules because the formulation of the reaction mixture does not support the formation of reverse transcription products prior to activation of the reverse transcriptase.

US Patent:

20120322057, Dec 20, 2012

Filed:

Jul 3, 2012

Appl. No.:

13/540935

Inventors:

Stephen P. HENDRICKS - Los Gatos CA, US
Michael PHELAN - Hayward CA, US
Marian PERIS - Belmont CA, US
Cheng-Yao CHEN - Carlsbad CA, US
Daniel MAZUR - San Diego CA, US
Xinzhan PENG - Carlsbad CA, US
Amy CASTILLO - Houston TX, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12N 9/12
C12Q 1/68
C12N 1/00
C12P 19/34
C12N 15/54
C12N 15/63

US Classification:

435 61, 435194, 536 232, 435243, 435 915

Abstract:

Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

US Patent:

20130005020, Jan 3, 2013

Filed:

Aug 31, 2012

Appl. No.:

13/600416

Inventors:

Marian PERIS - Belmont CA, US
Michael PHELAN - Hayward CA, US
Barnett B. ROSENBLUM - San Jose CA, US
Stephen P. HENDRICKS - Los Gatos CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12N 9/12

US Classification:

435194

Abstract:

Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

US Patent:

20230055565, Feb 23, 2023

Filed:

Sep 26, 2022

Appl. No.:

17/935406

Inventors:

- Carlsbad CA, US
Michael Phelan - Millbrae CA, US
Marian Peris - San Mateo CA, US
Cheng-Yao Chen - San Diego CA, US
Daniel Mazur - San Diego CA, US
Xinzhan Peng - Carlsbad CA, US
Amy Castillo - Houston TX, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12Q 1/6869
C07H 19/20
C12N 9/12
C12N 9/96
C12Q 1/6818
G01N 33/58
G01N 21/64

Abstract:

Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

US Patent:

20200231948, Jul 23, 2020

Filed:

Mar 20, 2020

Appl. No.:

16/825788

Inventors:

- Carlsbad CA, US
Cheng-Yao CHEN - Eugene OR, US
Guobin LUO - Oceanside CA, US
Michael PREVITE - Carlsbad CA, US
Jamshid TEMIROV - Germantown TN, US
Theo NIKIFOROV - Carlsbad CA, US
Zhaohui ZHOU - San Ramon CA, US
Hongye SUN - Belmont CA, US
Yufang WANG - San Carlos CA, US
Stefanie Yukiko NISHIMURA - Mountain View CA, US
Hongyi WANG - Pearland TX, US
Marian PERIS - Dublin CA, US
Barnett ROSENBLUM - San Jose CA, US
Michael PHELAN - Millbrae CA, US

International Classification:

C12N 9/12
C12Q 1/6827
C12Q 1/6869
C12Q 1/6872
C12Q 1/6804
C12Q 1/6818
C12Q 1/68

Abstract:

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.