Maria Kristina Dulay

US Patent:

6866785, Mar 15, 2005

Filed:

Aug 13, 2001

Appl. No.:

09/929275

Inventors:

Richard N. Zare - Stanford CA, US
Maria T. Dulay - Sunnyvale CA, US
Joselito P. Quirino - Stanford CA, US
Bryson Bennett - Murray UT, US

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Stanford CA

International Classification:

B01D015/08

US Classification:

210656, 210635, 2101982, 210658, 2101983

Abstract:

A separation column and a method of making the separation column are provided. The separation column includes a separation channel and fritless separation medium in the channel. The separation medium includes a porous matrix, and the porous matrix includes a metal organic polymer, such as a photopolymer. The separation medium can be used to separate a sample of analytes.

US Patent:

6875348, Apr 5, 2005

Filed:

Apr 16, 2002

Appl. No.:

10/124654

Inventors:

Richard N. Zare - Stanford CA, US
Maria T. Dulay - Sunnyvale CA, US
Joselito P. Quirino - Sunnyvale CA, US
Bryson D. Bennett - Murray UT, US

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Stanford CA

International Classification:

B01D015/08

US Classification:

2101982, 2101983, 210635, 210656, 210658

Abstract:

A separation column and a method of preparing the separation column are provided. The separation column includes a separation channel and a porous matrix in the channel. The porous matrix includes a metal organic polymer, such as a photopolymer. The porous matrix can be a separation medium adapted to separate a sample of analytes or a frit adapted to retain a separation medium in the channel.

US Patent:

6884346, Apr 26, 2005

Filed:

Oct 15, 2001

Appl. No.:

09/978515

Inventors:

Richard N. Zare - Stanford CA, US
Maria T. Dulay - Sunnyvale CA, US
Joselito P. Quirino - Stanford CA, US
Bryson Bennett - Murray UT, US

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Stanford CA

International Classification:

B01D015/08

US Classification:

2101982, 2101983, 210635, 210656, 210658

Abstract:

A separation column and a method of making the separation column are provided. The separation column includes a separation channel and a separation medium in the channel. The separation medium includes a porous matrix, and the porous matrix includes a support and a stationary phase. The support includes a metal organic polymer, such as a photopolymer, and the stationary phase includes a bonded phase. The separation medium can be used to separate a sample of analytes.

US Patent:

6986841, Jan 17, 2006

Filed:

Sep 29, 2003

Appl. No.:

10/674652

Inventors:

Richard N. Zare - Stanford CA, US
Maria T. Dulay - Sunnyvale CA, US
Jing-Ran Chen - Shaumburg IL, US

International Classification:

B01D 15/08

US Classification:

2101982, 210635, 210656, 96101

Abstract:

A separation column is provided with a photopolymer component which, when irradiated, causes controlled porosity polymerization. A particularly preferred embodiment is wherein the separation medium is retained by a photopolymer frit, which can be reliably and reproducibly generated with controlled porosity.

US Patent:

20020079257, Jun 27, 2002

Filed:

Nov 13, 2001

Appl. No.:

10/008482

Inventors:

Richard Zare - Stanford CA, US
Maria Dulay - Sunnyvale CA, US
Jing-Ran Chen - Shaumburg IL, US

International Classification:

B01D015/08

US Classification:

210/198200, 210/656000

Abstract:

A separation column is provided with a photopolymer component which, when irradiated, causes controlled porosity polymerization. A particularly preferred embodiment is wherein the separation medium is retained by a photopolymer frit, which can be reliably and reproducibly generated with controlled porosity.

US Patent:

20050127002, Jun 16, 2005

Filed:

Dec 12, 2003

Appl. No.:

10/734998

Inventors:

Richard Zare - Stanford CA, US
Maria Dulay - Sunnyvale CA, US
Quentin Baca - Albuquerque NM, US
Gabriele Trapp - Goeppingen, DE

International Classification:

B01D015/08

US Classification:

210656000, 210635000, 435004000, 435007100

Abstract:

A method that comprises providing a polymerized sol-gel material (PSG) and linking an enzyme to a surface of the PSG via covalent linkage is provided. The surface of the PSG is derivatized with a linker that comprises a functional group for linking itself to the surface of the PSG and a functional group for linking itself with then enzyme. The linked-enzyme PSG, or microreactor, is an effective means of at least partially digesting a substrate, such as a biological substrate. The activity of the enzyme of the microreactor may be significantly enhanced, up to 200-fold for example, relative to the activity of the enzyme free of the microreactor. The microreactor is thus an effective vehicle for digesting a substrate such as a biomolecule, a protein, an oligonucleotide, a peptide, a steroid, and/or an organic acid, after which, any remaining substrate and one or more digestion product(s) may be separated and detected. Microreactors and integrated devices that incorporate microreactors, such as columns, pipet tips, wells, and well-plates, are also provided.

US Patent:

20070141550, Jun 21, 2007

Filed:

Dec 13, 2004

Appl. No.:

10/582646

Inventors:

Richard Zare - Stanford CA, US
Maria Dulay - Sunnyvale CA, US
Quentin Baca - Albuquerque NM, US
Gabriele Trapp - Coeppingen, DE

International Classification:

C12Q 1/68
G01N 33/53
C12Q 1/00
C12M 3/00
B05D 3/02

US Classification:

435004000, 435007100, 435287200, 427002110, 977924000, 435006000

Abstract:

A method that comprises providing a polymerized sol-gel material (PSG) and linking an enzyme to a surface of the PSG via covalent linkage is provided. The surface of the PSG is derivatized with a linker that comprises a functional group for linking itself to the surface of the PSG and a functional group for linking itself with the enzyme. The linked-enzyme PSG, or microreactor, is an effective means of at least partially digesting a substrate, such as a biological substrate. The activity of the enzyme of the microreactor may be significantly enhanced, up to 200-fold for example, relative to the activity of the enzyme free of the microreactor. The microreactor is thus an effective vehicle for digesting a substrate such as a biomolecule, a protein, an oligonucleotide, a peptide, a steroid, and/or an organic acid, after which, any remaining substrate and one or more digestion product(s) may be separated and detected. Microreactors and integrated devices that incorporate microreactors, such as columns, pipet tips, wells, and well-plates, are also provided.

US Patent:

20070284308, Dec 13, 2007

Filed:

Jun 12, 2006

Appl. No.:

11/451711

Inventors:

Richard N. Zare - Stanford CA, US
Maria T. Dulay - Sunnyvale CA, US

International Classification:

B01D 15/08

US Classification:

210656, 204456, 435 4

Abstract:

A method that comprises providing a polymerized sol-gel material (PSG) and linking an enzyme to a surface of the PSG via covalent linkage is provided. The surface of the PSG is derivatized with a linker that comprises a functional group for linking itself to the surface of the PSG and a functional group for linking itself with the enzyme. The linked-enzyme PSG, or microreactor, is an effective means of at least partially digesting a substrate, such as a biological substrate. The activity of the enzyme of the microreactor may be significantly enhanced, up to 200-fold to over 2000-fold, for example, relative to the activity of the enzyme free of the microreactor. The microreactor is thus an effective vehicle for digesting a substrate such as a biomolecule, a protein, an oligonucleotide, a peptide, a steroid, and/or an organic acid, after which, any remaining substrate and one or more digestion product(s) may be separated and detected. Microreactors and integrated devices that incorporate microreactors, such as chips, columns, pipet tips, wells, and well-plates, are also provided.