Lucy Ellen Desjardin

US Patent:

6204026, Mar 20, 2001

Filed:

Nov 5, 1997

Appl. No.:

8/964877

Inventors:

Lucy Ellen DesJardin - Little Rock AR
Mac Donald Cave - Little Rock AR
Kathleen Davis Eisenach - Little Rock AR

Assignee:

The Board of Trustees of the University of Arkansas - Little Rock AR

International Classification:

C12Q 168
C07M 2104

US Classification:

435 912

Abstract:

The present invention provides primers which can be used for M. tuberculosis complex-specific detection of. alpha. -antigen DNA in a diagnostic assay performed on clinical specimens or in a culture-confirmation assay following growth of the organism in vitro. These primers and probes can also be employed in a reverse transcriptase-mediated amplification system for M. tuberculosis complex. alpha. -antigen mRNA. Such an assay provides a means by which to determine the viability of M. tuberculosis complex organisms either in clinical specimens or when grown in culture. Also described are methods for the detection of the products of amplification with a radiolabeled probe by chemiluminescent assay or fluorescence polarization analysis.

US Patent:

61565083, Dec 5, 2000

Filed:

Nov 5, 1997

Appl. No.:

8/964685

Inventors:

Patricia Anne Spears - Raleigh NC
Tobin James Hellyer - Little Rock AR
Lucy Ellen DesJardin - Little Rock AR
Mac Donald Cave - Little Rock AR
Kathleen Davis Eisenach - Little Rock AR

International Classification:

C12Q 168
C12P 1934
C07H 2104

US Classification:

435 6

Abstract:

The present invention provides primers which can be used for M. tuberculosis complex-specific detection of. alpha. -antigen DNA in a diagnostic assay performed on clinical specimens or in a culture-confirmation assay following growth of the organism in vitro. These primers and probes can also be employed in a reverse transcriptase-mediated amplification system for M. tuberculosis complex. alpha. -antigen mRNA. Such an assay provides a means by which to determine the viability of M. tuberculosis complex organisms either in clinical specimens or when grown in culture. The specific DNA or mRNA target region can be amplified using SDA, PCR, LCR, Nucleic Acid Sequence Based Amplification (NASBA), Self-sustained Sequence Replication (3SR) or Q. beta. Replicase-mediated systems. Also described are methods for the detection of the products of amplification with a radiolabeled probe by chemiluminescent assay or fluorescence polarization analysis.