Louis T Graziano

US Patent:

6410275, Jun 25, 2002

Filed:

Oct 18, 1999

Appl. No.:

09/420139

Inventors:

Bryan Kluttz - Norwell MA
Lawrence Burg - Framingham MA
Garry Tegeler - Hazelwood MO
Louis Graziano - Rockland MA
Christopher Cotter - Charlestown MA
Michel Guy - Scituate MA
James Greer - Rockland MA
Geoff McKinley - Duxbury MA
Luigi Catanzariti - Duxbury MA
James Clement Bishop - Columbia MO
Bruno Colin - Marcy lEtoile, FR
Cécile Paris - Lyons, FR
Thomas Wang - Lexington MA
Michael Morin - Dedham MA

Assignee:

bioMerieux, Inc. - Hazelwood MO

International Classification:

C12P 1934

US Classification:

435 911, 435 912, 4352851, 4352872, 4352873, 4352876, 4352885, 422 64, 422101, 422102, 935 78, 935 87, 935 88

Abstract:

A unit dose test device for a nucleic acid amplification reaction has the form of a elongate disposable test strip. The test strip includes a dual-chamber reaction vessel pre-loaded with nucleic acid amplification reaction reagents, and a plurality of wells for processing a reaction occurring in the reaction vessel.

US Patent:

6429007, Aug 6, 2002

Filed:

Oct 18, 1999

Appl. No.:

09/420140

Inventors:

Bryan W. Kluttz - Norwell MA
Geoff A. McKinley - Duxbury MA
Fabio Gennari - Florence, IT
Michel Guy - Scituate MA
Christopher Cotter - Charlestown MA
Luigi Catanzariti - Duxbury MA
Louis Graziano - Rockland MA
Bruno Colin - Marcy lEtoile, FR
Cecile Jaravel - Lyons, FR
Jacques Dachaud - Besancon, FR

Assignee:

bioMérieux, Inc. - Hazelwood MO

International Classification:

C12M 136

US Classification:

4352865, 4352866, 4352872, 4352873, 4352876, 4352885, 422 64

Abstract:

An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e. g. , primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e. g. , an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

US Patent:

6949376, Sep 27, 2005

Filed:

May 7, 2002

Appl. No.:

10/141049

Inventors:

Bryan W. Kluttz - Norwell MA, US
Geoff A. McKinley - Duxbury MA, US
Fabio Gennari - Florence, IT
Michel Guy - Scituate MA, US
Christopher Cotter - Charlestown MA, US
Luigi Catanzariti - Duxbury MA, US
Louis Graziano - Rockland MA, US
Bruno Colin - Marcy l'Etoile, FR
Cecile Jaravel - Lyons, FR
Jacques Dachaud - Besancon, FR

Assignee:

bioMerieux, Inc. - Durham NC

International Classification:

C12M001/36

US Classification:

4352865, 4352886, 4352872, 4352876, 422 681

Abstract:

An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e. g. , primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e. g. , an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

US Patent:

7214529, May 8, 2007

Filed:

Jul 12, 2005

Appl. No.:

11/180806

Inventors:

Bryan W. Kluttz - Norwell MA, US
Geoff A. McKinley - Duxbury MA, US
Fabio Gennari - Florence, IT
Michel Guy - Scituate MA, US
Christopher Cotter - Charlestown MA, US
Luigi Catanzariti - Duxbury MA, US
Louis Graziano - Rockland MA, US
Bruno Colin - Marcy l'Etoile, FR
Cecile Jaravel - Lyons, FR
Jacques Dachaud - Besancon, FR

Assignee:

bioMérieux, Inc. - Durham NC

International Classification:

C12M 1/36

US Classification:

4352865, 4352872, 4352873, 4352876

Abstract:

An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e. g. , primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e. g. , an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

US Patent:

7807449, Oct 5, 2010

Filed:

May 22, 2006

Appl. No.:

11/438938

Inventors:

Bryan W. Kluttz - Norwell MA, US
Geoff A. McKinley - Duxbury MA, US
Fabio Gennari - Florence, IT
Michel Guy - Scituate MA, US
Christopher Cotter - Charlestown MA, US
Luigi Catazariti - Duxbury MA, US
Louis Graziano - Rockland MA, US
Bruno Colin - Marcy l'Etoile, FR
Cecile Paris - Marcy l'Etoile, FR
Jacque Dachaud - Besancon, FR

Assignee:

bioMerieux, Inc. - Durham NC

International Classification:

C12M 1/34
C12M 3/00
C12M 1/36
C12M 1/38

US Classification:

4352873, 4352865, 4352866, 4352872, 4352883, 4352884, 4352885, 4353052, 4352887, 422102, 422103

Abstract:

An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e. g. , primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e. g. , an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

US Patent:

20110020921, Jan 27, 2011

Filed:

Sep 24, 2010

Appl. No.:

12/924351

Inventors:

Bryan W. Kluttz - Norwell MA, US
Geoff A. McKinley - Duxbury MA, US
Fabio Gennari - Florence, IT
Michel Guy - Scituate MA, US
Christopher Cotter - Charlestown MA, US
Luigi Catanzariti - Duxbury MA, US
Louis Graziano - Rockland MA, US
Bruno Colin - Marcy L'Etoile, FR
Cecile Paris - Lyon, FR
Jacques Dachaud - Besancon, FR

Assignee:

bioMerieux, Inc. - Durham NC

International Classification:

C12M 1/34

US Classification:

4352872

Abstract:

An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

US Patent:

20130273644, Oct 17, 2013

Filed:

Mar 13, 2013

Appl. No.:

13/799275

Inventors:

Geoff A. McKinley - Duxbury MA, US
Fabio Gennari - Florence, IT
Michel Guy - Scituate MA, US
Christopher Cotter - Charlestown MA, US
Luigi Catazariti - Duxbury MA, US
Louis Graziano - Rockland MA, US
Bruno Colin - Marcy L'Etoile, FR
Cecile Paris - Lyon, FR
Jacque Dachaud - Besancon, FR

Assignee:

BIOMERIEUX, INC. - DURHAM NC

International Classification:

C12Q 1/68

US Classification:

4352872

Abstract:

An instrument for conducting nucleic acid amplification reactions in a disposable test device is provided. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

US Patent:

57861820, Jul 28, 1998

Filed:

May 2, 1997

Appl. No.:

8/850207

Inventors:

Luigi Catanzariti - Duxbury MA
Bryan W. Kluttz - Norwell MA
Geoff A. McKinley - Duxbury MA
Arthur L. Garland - Carver MA
Louis Graziano - Rockland MA
James G. Moe - Franklin MA
Marcela Vera-Garcia - Framingham MA
James Clement Bishop - Columbia MO
David Chastain - Acton MA
Fabio Gennari - Florence, IT

Assignee:

bioMerieux Vitek, Inc. - Hazelwood MO

International Classification:

C12P 1934
C12M 300
G01N 2100
C12N 1500

US Classification:

435 911

Abstract:

A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix, a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber, the fluid channel is opened to allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction. A station is described for processing test strips incorporating the reaction vessels. The station includes temperature and vacuum control subsystems to maintain proper temperatures in the reaction vessel and effectuate the transfer of the fluid from one chamber to the other in an automated fashion without human intervention.