Linda B Jacobsen

US Patent:

57534853, May 19, 1998

Filed:

Jan 24, 1996

Appl. No.:

8/590957

Inventors:

Francis E. Dwulet - Greenwood IN
Bernice B. Ellis - Greenfield IN
John F. Gill - Indianapolis IN
Linda B. Jacobsen - Indianapolis IN
Marilyn E. Smith - McCordsville IN
David G. Waters - Indianapolis IN

Assignee:

Boehringer Mannheim Corporation - Indianapolis IN

International Classification:

C12N 952
C12N 950

US Classification:

435220

Abstract:

A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cell clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.

US Patent:

59898887, Nov 23, 1999

Filed:

Mar 13, 1998

Appl. No.:

9/042295

Inventors:

Francis E. Dwulet - Greenwood IN
Bernice B. Ellis - Greenfield IN
John F. Gill - Indianapolis IN
Linda B. Jacobsen - Indianapolis IN
Marilyn E. Smith - McCordsville IN
David G. Waters - Indianapolis IN

Assignee:

Roche Diagnostics Corporation - Indianapolis IN

International Classification:

C12N 952
C12N 950

US Classification:

435220

Abstract:

A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cell clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.