Liang X Shi

Author:

Liang Shi

ISBN #:

0773471448

US Patent:

7202022, Apr 10, 2007

Filed:

Jun 29, 2001

Appl. No.:

09/896324

Inventors:

Bi-Yu Li - San Diego CA, US
Xun Wang - San Diego CA, US
Liang Shi - San Diego CA, US

Assignee:

Syngenta Participations AG - Basel

International Classification:

C12Q 1/68
C12P 19/34
C07H 19/00
C07H 21/04

US Classification:

435 6, 435 9121, 536 221, 536 2433

Abstract:

The invention relates to a new method for sequence-specific identification, separation and quantitative measurement of nucleic acid fragments. The invention is based on the use of restriction endonucleases that have degenerate bases in their recognition or cleavage sequence. The method has broad applications, including DNA fingerprinting, differential display of mRNA, mutation and polymorphism identification, diagnosis and drug screening.

US Patent:

7300751, Nov 27, 2007

Filed:

Sep 6, 2002

Appl. No.:

10/236363

Inventors:

Bi-Yu Li - San Diego CA, US
Xun Wang - San Diego CA, US
Liang Shi - San Diego CA, US

Assignee:

Syngenta Participations AG - Basel

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 6, 435 912, 536 231, 536 2433

Abstract:

Methods are provided for identifying genetic markers and for determining methylation patterns using restriction endonucleases having degenerate recognition or cleavage sequences.

US Patent:

20020034753, Mar 21, 2002

Filed:

May 14, 2001

Appl. No.:

09/863527

Inventors:

Li Yang - San Diego CA, US
Xun Wang - San Diego CA, US
Tong Zhu - San Diego CA, US
Liang Shi - San Diego CA, US

International Classification:

C12Q001/68
C12Q001/70

US Classification:

435/006000, 435/005000

Abstract:

Currently, three technologies are utilized for analysis of gene expression: hybridization-based technologies, PCR-based technologies, and sequence-based technologies. The present invention provides a method for analyzing the presence and/or amount of a specific nucleic acid using a solid support and a capture probe complementary to a region of a target nucleic acid, and polymerizing a labeled extension complementary to the target nucleic acid. The invention provides a method of analysis of all types of nucleic acids, and can be used to study multiple genes in a single assay using different capture probes conjugated to different class of microspheres that can be mixed in any desired combination.

US Patent:

20020146839, Oct 10, 2002

Filed:

Jan 28, 2002

Appl. No.:

10/058963

Inventors:

Andras Guttman - San Diego CA, US
Liang Shi - San Diego CA, US
Xun Wang - San Diego CA, US

International Classification:

G01N027/26
G01N027/447
G01N001/18

US Classification:

436/177000, 204/601000, 204/603000, 204/604000, 204/605000, 204/615000, 204/451000, 204/452000, 204/453000, 204/455000, 204/465000, 436/161000, 422/070000, 436/178000, 422/101000, 422/082010, 435/287100, 435/287200, 435/288600

Abstract:

A method of injecting, isolating and separating mixed analytes from one or more samples. The method includes collecting successive fractions from each of a plurality of samples at discrete points in time. Fractions may be analyzed at the time of collecting, or later, using one or more detector systems. In one embodiment, a processor controls the elutions of fractions by modulating the migration field in a separation pathway. The processor also controls distribution of the fraction into a particular collection well of a plurality of collection wells.

US Patent:

20030022192, Jan 30, 2003

Filed:

Jan 23, 2002

Appl. No.:

10/055109

Inventors:

Liang Shi - San Diego CA, US
Xun Wang - San Diego CA, US
Bi-Yu Li - San Diego CA, US

International Classification:

C12Q001/68
G01N033/53
C12P019/34

US Classification:

435/006000, 435/007100, 435/091200

Abstract:

Provided are novel methods for the production of nucleic acid libraries having reduced redundancy. Also provided are methods for determination of changes in RNA expression associated with pathological conditions, and physiological or developmental state. Additional aspects provided non-redundant tags or probes produced by the disclosed methods and microarrays incorporating such tags.

US Patent:

20030082584, May 1, 2003

Filed:

Jun 28, 2002

Appl. No.:

10/187039

Inventors:

Liang Shi - San Diego CA, US
Bi-Yu Li - San Diego CA, US
Xun Wang - San Diego CA, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

Disclosed herein are novel methods for use in the analysis of nucleic acids. Uses disclosed include polynucleotide expression analysis and detection of single nucleotide polymorphisms. Also provided are diagnostic methods and kits for conducting the disclosed methods.

US Patent:

20030170695, Sep 11, 2003

Filed:

Dec 27, 2002

Appl. No.:

10/330774

Inventors:

Liang Shi - San Diego CA, US
Bi-Yu Li - San Diego CA, US
Xun Wang - San Diego CA, US

International Classification:

C12Q001/68
C07H021/04
C12P019/34

US Classification:

435/006000, 435/091200, 536/024300

Abstract:

Disclosed herein are novel methods for use in the analysis of nucleic acids. Uses disclosed include polynucleotide expression analysis, detection of single nucleotide polymorphisms, detection of pathogens, and detection of genetically modified cells and organisms. The method can be practiced using purified preparations of nucleic acids or unpurified cell lysates. Also provided are diagnostic methods and kits for conducting the disclosed methods.

US Patent:

20030190663, Oct 9, 2003

Filed:

May 2, 2003

Appl. No.:

10/428697

Inventors:

Li Yang - San Diego CA, US
Xun Wang - Research Triangle Park NC, US
Tong Zhu - Research Triangle Park NC, US
Liang Shi - San Diego CA, US

Assignee:

Syngenta Participations AG

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

Currently, three technologies are utilized for analysis of gene expression: hybridzation-based technologies, PCR-based technologies, and sequence-based technologies The present invention provides a method for analyzing the presence and/or amount of a specific nucleic acid using a solid support and a capture probe complementary to a region of a target nucleic acid, and polymerizing a labeled extension complementary to the target nucleic acid The invention provides a method of analysis of all types of nucleic acids, and can be used to study multiple genes in a single assay using different capture probes conjugated to different class of microspheres that can be mixed in any desired combination.