Leigh R Anderson

US Patent:

20120309027, Dec 6, 2012

Filed:

May 31, 2012

Appl. No.:

13/484357

Inventors:

Leigh ANDERSON - Washington DC, US
Detlev SUCKAU - Grasberg, DE

Assignee:

BRUKER DALTONIK GMBH - Bremen

International Classification:

G01N 27/62

US Classification:

435 721, 436501, 435 71

Abstract:

A method for determining the concentration ratio in a sample of a target peptide to a reference peptide that is chemically identical with the target peptide, but labeled by isotopes, acquires mass spectra of the target and reference peptides. One of a plurality of families of superimposed bell-shaped curves which is a best fit to ion current peak groups of the target and reference peptides in the mass spectra is determined by varying parameters of the families. In each family, each bell-shaped curve has a predetermined height, the curves have fixed distances from each other and the relative curve heights and curve distances in the families are individually calculated from an elemental composition of the peptides and an isotope abundance distribution of elements composing the peptides, taking into account purity of the isotopes. The concentration ratio is then determined from the parameters of the best fit.

US Patent:

20170138955, May 18, 2017

Filed:

Jan 27, 2017

Appl. No.:

15/418320

Inventors:

Leigh ANDERSON - Washington DC, US
Detlev SUCKAU - Grasberg, DE

Assignee:

Bruker Daltonik GmbH - Bremen
Anderson Forschung Group LLC - Washington DC

International Classification:

G01N 33/68

Abstract:

A method for determining the concentration ratio in a sample of a target peptide to a reference peptide that is chemically identical with the target peptide, but labeled by isotopes, acquires mass spectra of the target and reference peptides. One of a plurality of families of superimposed bell-shaped curves which is a best fit to ion current peak groups of the target and reference peptides in the mass spectra is determined by varying parameters of the families. In each family, each bell-shaped curve has a predetermined height, the curves have fixed distances from each other and the relative curve heights and curve distances in the families are individually calculated from an elemental composition of the peptides and an isotope abundance distribution of elements composing the peptides, taking into account purity of the isotopes. The concentration ratio is then determined from the parameters of the best fit.