Kirk D Hartel

US Patent:

20060134644, Jun 22, 2006

Filed:

Oct 28, 2004

Appl. No.:

10/977347

Inventors:

Kirk Hartel - West Fargo ND, US
Gregory Gillispie - Fargo ND, US
Mark Pavicic - Fargo ND, US

International Classification:

C12Q 1/68
C12M 1/34

US Classification:

435006000, 435287200

Abstract:

This invention relates to apparatus and methods to detect a target analyte in a test sample by forming a fluorescent complex comprising the target analyte and a probe. The fluorescence decay and/or lifetime changes upon complex formation. The apparatus includes a pulsed light source and a digitizer to measure fluorescent decay and/or lifetime of the fluorophore in the complex

US Patent:

20050136448, Jun 23, 2005

Filed:

Sep 29, 2004

Appl. No.:

10/954942

Inventors:

Kirk Hartel - West Fargo ND, US
Mark Pavicic - Fargo ND, US
Gregory Gillispie - Fargo ND, US

Assignee:

Dakota Technologies, Inc. - Fargo ND

International Classification:

C12Q001/68
C12M001/34
G03C005/16

US Classification:

435006000, 435287200, 250581000

Abstract:

Apparatus and methods are provided to monitor hybridization of a probe polynucleotide for detection and analysis of a target polynucleotide in a test sample. A single-stranded probe polynucleotide is attached to an identifiable region on a substrate and allowed to hybridize to a complementary target polynucleotide that may be present in a test sample. A fluorophore noncovalently interacts with the double-stranded polynucleotides that may be formed. The fluorescence decay or lifetime of the fluorophore associated with the double-stranded polynucleotide is different from the fluorescence decay or lifetime of the fluorophore when it is associated with a single-stranded polynucleotide. The detection of double-stranded polynucleotides at a particular region is an indication that the target polynucleotide is present in the test sample.