Joseph Thomas Ippoliti - Woodbury MN, US Katherine E. Olson - Lakeland MN, US
International Classification:
G01N 33/53
US Classification:
435 79
Abstract:
Disclosed is an assay for detecting an analyte. The method includes the steps of contacting a solution suspected of containing the analyte with capture antibodies specific for the analyte, wherein analyte contained in the solution is captured by the capture antibodies. Then contacting the capture antibodies with a solution containing the analyte attached to an alcohol oxidase (AOX) enzyme, to yield captured, labeled analyte. Then contacting the capture antibodies with a reagent mixture that generates a first signal proportional to the captured, labeled analyte and quantifying the first signal. And then measuring concentration of the analyte in the unknown sample by comparing the first signal to standard curve of signals. The assay can be implemented in an ELISA format.
Isbn (Books And Publications)
Standing Tall: The Lifeway Of Kathryn Jones Harrison
Ms. Olson works in Longmont, CO and 1 other location and specializes in Urology. Ms. Olson is affiliated with Avista Adventist Hospital, Boulder Community Hospital, Longmont United Hospital and St Joseph Hospital.