Karen Rae Caris

US Patent:

52178730, Jun 8, 1993

Filed:

Sep 9, 1991

Appl. No.:

7/756444

Inventors:

Karen R. Caris - Camarillo CA
Ivan E. Modrovich - Camarillo CA

International Classification:

C12Q 160

US Classification:

435 11

Abstract:

There is provided a stable cholesterol assay composition which comprises an aqueous solution of at least one bile acid or salt thereof being present in an amount of up to about 5 mM; a nonionic surfactant present in an amount of from about 0. 15 to about 1. 5 percent volume by volume; a buffer in a concentration of from 0 to about 65 mM; and cholesterol oxidase in a concentration of at least about 0. 1 KIU/l. Solution pH is from about 5. 5 to about 8. 5. Addition of cholesterol esterase, phenol, peroxidase and 4-aminoantipyrine provides a total cholesterol chromogen system.

US Patent:

50473271, Sep 10, 1991

Filed:

Jan 3, 1989

Appl. No.:

7/294896

Inventors:

Karen R. Caris - Camarillo CA
Ivan E. Modrovich - Camarillo CA

International Classification:

C12Q 160

US Classification:

435 11

Abstract:

There is provided a stable cholesterol assay composition which comprises an aqueous solution of at least one bile acid or salt thereof being present in an amount of up to about 5 mM; a nonionic surfactant present in an amount of from about 0. 15 to about 1. 5 percent volume by volume; a buffer in a concentration of from 0 to about 65 mM; and cholesterol oxidase in a concentration of at least about 0. 1 kIU/l. Solution pH is from about 5. 5 to about 8. 5. Addition of cholesterol esterase, phenol, peroxidase and 4-aminoantipyrine provides a total cholesterol chromogen system.