Judith K Atwood

Author:

Judith G. Atwood

ISBN #:

0397541872

Author:

Judith G. Atwood

ISBN #:

0397541880

US Patent:

20020192808, Dec 19, 2002

Filed:

Aug 9, 2002

Appl. No.:

10/216620

Inventors:

Michael Gambini - Bolton MA, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US
Eugene Young - Foster City CA, US
Edward Lakatos - Bethel CT, US
Anthony Cerrone - New Haven CT, US

International Classification:

C12M001/34

US Classification:

435/287200, 435/288700

Abstract:

An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

US Patent:

20030148505, Aug 7, 2003

Filed:

Feb 20, 2003

Appl. No.:

10/370846

Inventors:

Michael Gambini - Bolton MA, US
John Atwood - Redding CT, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US
Eugene Young - Foster City CA, US
Edward Lakatos - Bethel CT, US
Anthony Cerrone - New Haven CT, US

International Classification:

C12M001/34

US Classification:

435/287200, 435/288400, 435/288700

Abstract:

An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

US Patent:

20050084957, Apr 21, 2005

Filed:

Aug 30, 2004

Appl. No.:

10/929961

Inventors:

John Atwood - West Redding CT, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US
Albert Mossa - Trumbull CT, US
Lisa Goven - Bridgeport CT, US
Fenton Williams - Brookfield CT, US
Timothy Woudenberg - Bethel CT, US
Marcel Margulies - Scarsdale NY, US
Robert Ragusa - Newton CT, US
Richard Leath - Berkley CA, US
Clive Miles - San Raphael CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12M001/24

US Classification:

435304100

Abstract:

A sample tube including a cylindrical part and a conical part is described. The cylindrical part can include, a first wall portion having a first wall portion thickness, a second wall portion having a second wall portion thickness, and a shoulder including a planar first surface and a conically beveled second surface disposed between the first wall portion and the second wall portion. The first wall portion can be disposed closer to the planar first surface than the conically beveled second surface. The conical part can be shaped as a frustrum of a cone having a conical part wall thickness. The frustrum can include a wider-end and a narrower-end. The wider-end of the frustrum can be in contact with the second wall portion of the cylindrical part. At least one of the first wall portion thickness and the second wall portion thickness can be greater than the conical part wall thickness.

US Patent:

20060128009, Jun 15, 2006

Filed:

Jan 17, 2006

Appl. No.:

11/333483

Inventors:

Anthony Cerrone - New Haven CT, US
Edward Lakatos - Bethel CT, US
Michael Gambini - Bolton MA, US
Eugene Young - Foster City CA, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US

International Classification:

C12M 1/34

US Classification:

435288700, 435288400, 435303100

Abstract:

An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

US Patent:

20060199259, Sep 7, 2006

Filed:

Apr 18, 2006

Appl. No.:

11/406148

Inventors:

Anthony Cerrone - Northford CT, US
Edward Lakatos - Bethel CT, US
Michael Gambini - Monroe CT, US
Eugene Young - Foster City CA, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US
John Atwood - Redding CT, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12M 1/34

US Classification:

435288700, 435287200

Abstract:

An instrument for monitoring replication of DNA is provided.

US Patent:

20070148761, Jun 28, 2007

Filed:

Feb 13, 2007

Appl. No.:

11/705856

Inventors:

Anthony Cerrone - New Haven CT, US
Edward Lakatos - Bethel CT, US
Michael Gambini - Monroe CT, US
Eugene Young - Foster City CA, US
Judith Atwood - Redding CT, US
John Atwood - Redding CT, US
Susan Stone - Charlotte VT, US

International Classification:

C12M 1/34

US Classification:

435287200, 435288700

Abstract:

An optical instrument monitors PCR replication of DNA in a reaction apparatus having vials of reaction ingredients. A beam splitter passes an excitation beam to the vials. An emission beam from the reaction ingredients can be passed by the beam splitter to a detector from which a processor can compute DNA concentration. A reference strip with a plurality of reference emitters can be provided which emits reference beams of different intensity, from which the processor can select an optimum emitter for compensating for drift. Exposure time can be automatically adjusted for keeping within optimum dynamic ranges of the detector and processor. A module of the beam splitter and associated optical filters can be associated with a selected dye, and can be replaceable for different dyes.

US Patent:

20070154939, Jul 5, 2007

Filed:

Feb 26, 2007

Appl. No.:

11/711416

Inventors:

Anthony Cerrone - New Haven CT, US
Edward Lakatos - Bethel CT, US
Michael Gambini - Monroe CT, US
Eugene Young - Foster City CA, US
John Atwood - Redding CT, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68
G06F 19/00
C12M 3/00

US Classification:

435006000, 435287200, 702020000

Abstract:

An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

US Patent:

20070238161, Oct 11, 2007

Filed:

May 17, 2007

Appl. No.:

11/804151

Inventors:

Anthony Cerrone - New Haven CT, US
Edward Lakatos - Bethel CT, US
Michael Gambini - Monroe CT, US
Eugene Young - Foster City CA, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12M 1/34
C12M 1/36

US Classification:

435286100, 435287200

Abstract:

An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.