Joye L. Bramble - Lansdale PA Fernando J. Muzzio - Spotswood NJ James A. Searles - Boulder CO
Assignee:
Merck & Co., Inc. - Rahway NJ Rutgers, The State University of New Jersey - New Brunswick NJ
International Classification:
C12M 302
US Classification:
435394
Abstract:
A method for enhancing the mixing of materials in a roller bottle by the introduction of controlled axial and cross-sectional flow perturbations is disclosed. The effectiveness of the method is demonstrated by achieving higher efficiency in cell culturing and virus propagation in roller bottles by introducing controlled flow perturbations during the process.
Method Of Improved Mixing Of A Varicella-Infected Cell Culture In Roller Bottles
Joye L. Bramble - Lansdale PA Fernando J. Muzzio - Spotswood NJ James A. Searles - Boulder CO
Assignee:
Merck & Co., Inc. - Rahway NJ
International Classification:
C12N 700
US Classification:
4352351
Abstract:
A method for enhancing the mixing of a varicella-infected cell culture in a roller bottle by the introduction of controlled cross-sectional flow perturbations in roller bottle rotation is disclosed. The effectiveness of the method is demonstrated by achieving higher efficiency in cell culturing and virus propagation in roller bottles by introducing controlled cross-sectional flow perturbations during the process.