Joseph J Wynne

US Patent:

20120066782, Mar 15, 2012

Filed:

Aug 31, 2011

Appl. No.:

13/222620

Inventors:

Mark R. PHILIPS - New York NY, US
Nicole FEHRENBACHER - New York NY, US
Joseph WYNNE - New York NY, US

Assignee:

NEW YORK UNIVERSITY - New York NY

International Classification:

C12Q 1/66
A01K 67/027
C12Q 1/02

US Classification:

800 18, 435 8, 435 29, 800 13, 800 14

Abstract:

The present invention relates to a kit for detection of association of a peripheral cellular membrane binding protein with cellular membranes in living cells and methods thereof. The kit includes a first nucleic acid construct comprising a first nucleic acid molecule encoding a first fusion protein comprising a peripheral cellular membrane binding protein or membrane binding domain thereof operatively coupled to DNA binding and transactivation domains of a naturally occurring or chimeric transcription factor and a first promoter operatively associated with the first nucleic acid molecule. A second nucleic acid construct comprises a second nucleic acid molecule encoding a reporter protein and a second promoter responsive to the DNA binding and transactivation domains of the first fusion protein. The second promoter is operatively associated with the second nucleic acid molecule. Activation of the second promoter results in expression of the reporter protein. Also disclosed is a transgenic non-human animal.