Carl Fuller - Berkeley Heights NJ, Joseph Szasz - Chardon OH,
Amersham Biosciences Corp - Piscataway NJ
435 6, 536 231, 536 243, 536 2433, 530350
Thermostable DNA polymerases both in native form and having single amino acid substitutions and optionally N-terminal deletions are disclosed. These polymerases exhibit a substantial improvement of DNA sequencing performance compared to Taq DNA polymerase. The instant DNA polymerases also possess improved salt tolerance.
Maria Davis - Twinsburg OH Joseph Szasz - Chesterland OH
Amersham Life Science, Inc. - Cleveland OH
C12Q 168 C07H 2104
Alkaline phosphatase from Thermus thermophilus has been isolated. The enzyme has a pH optimum of greater than 10. 5 and is stable to heating at 65. degree. C for 1 hour. The claimed invention relates to a method of detecting nucleic acids in a sample by providing a nucleic acid probe labelled with thermostable alkaline phosphatase from Thermus thermophilus; contacting the sample with said labelled nucleic acid; and detecting said nucleic acid in said sample by means of said thermostable alkaline phosphatase.