- Tarrytown NY, US John McWhirter - Hastings-on-Hudson NY, US Corey Momont - White Plains NY, US Caitlin L. Goshert - Boston MA, US Lynn Macdonald - Harrison NY, US Gregg S. Warshaw - Bayside NY, US Jose F. Rojas - Newburgh NY, US Ka-Man Venus Lai - Seattle WA, US David M. Valenzuela - Yorktown Heights NY, US Andrew J. Murphy - Croton-on-Hudson NY, US
Assignee:
REGENERON PHARMACEUTICALS, INC. - Tarrytown NY
International Classification:
C12N 15/66 C12N 15/64 C12N 15/10
Abstract:
Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.
Methods And Compositions For Assessing Crispr/Cas-Induced Recombination With An Exogenous Donor Nucleic Acid In Vivo
- Tarrytown NY, US Charleen Hunt - Dumont NJ, US Suzanne Hartford - Putnam Valley NY, US Jose Rojas - Newburgh NY, US David Frendewey - New York NY, US Brian Zambrowicz - Sleepy Hollow NY, US Andrew J. Murphy - Croton-on-Hudson NY, US
Methods and compositions are provided for assessing CRISPR/Cas-induced recombination of a target genomic locus with an exogenous donor nucleic acid in vivo or ex vivo. The methods and compositions employ non-human animals comprising a CRISPR reporter such as a genomically integrated CRISPR reporter for detecting and measuring CRISPR/Cas-induced repair of a coding sequence for a catalytically inactive reporter protein through recombination with an exogenous donor nucleic acid. Methods and compositions are also provided for making and using these non-human animals.
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Holy Name Medical Center 718 Teaneck Road, Teaneck, NJ 07666
Education:
Medical School UMDNJ Nj Med School Newark Graduated: 1993 Medical School St Vincent's Hospital Graduated: 1994 Medical School St Vincent's Hospital Graduated: 1997 Medical School Saint Vincent's Hospital Graduated: 1998
Dr. Jose A Arabia Rojas, San Juan PR - MD (Doctor of Medicine)
Mar 2012 to 2000 Franchise Owner, System 4 of The Carolina'sSuperb Custom Cleaning Tampa, FL Aug 1998 to Jan 2012 Director / Manager
Education:
Cleaning Management Institute Charlotte, NC 2012 to 2012 Certificate of Custodial Technician in CustodialCTI New York, NY 1983 to 1983 Certificate of Travel & Tourism in Travel Agent
Skills:
Window 7, Microsoft Office 2013, Fluent in English and Spanish.
Laboratory Corporation of America Manhattan, NY Jan 1999 to Dec 1999Quest Diagnostics Teterboro, NJ 1995 to Jan 1999Greenville Hospital Jersey City, NJ 1993 to 1995 Data Entry job specifically entering patient's laboratory results to the Database computerL. A. Gear Los Angeles, CA 1987 to 1993Miles Square Health Center Chicago, IL 1982 to 1987
Education:
Medical Careers Institute Chicago, IL Jul 1983 Phlebotomy and Hematology TechnicianUniversity of the East Manila 1976 Bachelor of Science in Biological ScienceMedical Technology Velez College Philippines 1976
Bellevue, Washington Columbus, Ohio San Juan, Puerto Rico Miami, Florida Hanau, Germany Enterprise, Alabama Tuzla, Bosnia Herzegovina Boston, Massachusetts
Work:
Solavei JPMorgan Chase & Co. Popular, Inc. McKinsey & Company United States Army
Education:
Harvard Business School, United States Military Academy
Tagline:
Passions: Family, Technology, Banking, Outdoors, Puerto Rico
Jose Rojas (Jr)
Lived:
Sammamish, Washington San Juan, Puerto Rico Powell, Ohio Florida Alabama Hanau, Germany
Work:
Eastlake High School - Student (2012)
Jose Rojas
Lived:
SAN JUAN, PUERTO RICO BRONX NEW YORK
Work:
ACTION LOCK & DOOR - MECHANIC/INSTALLER (2010)
Education:
Monroe College - BACHELORS IN HOSPITALITY
Relationship:
Single
About:
NAME IS JOSE RJAS AND IM A PUERTORRIQUEÑO. I LIVE IN THE BRONX AND I'M 39 YRS OLD. I HAVE TWO KIDS AND ONE GRANDCHILD. I WORK AS A LOCK SMITH IN YONKERS NEW YORK. IM FUN HONEST, FAITHFUL AND MOST...
Bragging Rights:
A GRANDFATHER AND A FATHER WITH TWO KIDS
Jose Rojas
Work:
Marketing and International Business
Education:
UNITEC - Lic. Mercadotecnia y Negocios Internacionales
About:
Sonrie a la Vida
Tagline:
Trata de ser una mejor persona cada dia
Bragging Rights:
Ingles, Español
Jose Rojas
Lived:
BROOKLYN, NEW YORK
Work:
D.S.N.Y. - SANMAN
About:
Grant me the serenity to accept the things i cannot change, the courage to change the things I cannot accept, and the wisdom to hide the bodies of those people i had to kill today because they piss...
Jose Rojas
Education:
San jose Obrero - Electronica, Divino Pastor - EGB, Ingenieria Tecnica Industrial - Electronica
About:
Mi sueño es ser locutor de radio, me conformo con ser electrónico, radioaficionado, auditor wireless y experto en excel, aunque llevo 20 años de gasolinero, y administro con mas pena que gloria www.so...
Tagline:
Conozco a los cojos sentaos
Jose Rojas
Work:
Sunburns - Tecnician Assembly
Education:
Hernando Navia Varon
Relationship:
In_civil_union
About:
Me gusta la musica de cualquier genero, especialmente la salsa, compartir y disfrutar buenos momentos con mis amigos y familiares.
Bragging Rights:
Estoy localizado en Erie PA, comparto mi hogar con tres niños, Damian Isaac y Dayana mi compañera Patty. Mis Otros Dos hijos en Cali Colombia Josselyn Y Bryam. Incha del mejor equipo del mundo El Deportivo Cali. y amante de la buena salsa oirla y bailarla.
Jose “Atomicogames” Rojas
Work:
Ejercito de Chile - CB0 Concripto
Education:
Duoc UC - Técnico en Electricidad y Automatización Industrial