John J Wyrick

US Patent:

7575869, Aug 18, 2009

Filed:

Aug 31, 2007

Appl. No.:

11/897841

Inventors:

John Wyrick - Pasadena CA, US
Richard A. Young - Weston MA, US
Bing Ren - La Jolla CA, US
Robert Francois - Boston MA, US
Itamar Simon - Brighton MA, US

Assignee:

Whitehead Institute for Biomedical Research - Cambridge MA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 912

Abstract:

The present invention relates to a method of identifying a region (one or more) of a genome of a cell to which a protein of interest binds. In the methods described herein, DNA binding protein of a cell is linked (e. g. , covalently crosslinked) to genomic DNA of a cell. The genomic DNA to which the DNA binding protein is linked is removed and combined or contacted with DNA comprising a sequence complementary to genomic DNA of the cell under conditions in which hybridization between the identified genomic DNA and the sequence complementary to genomic DNA occurs. Region(s) of hybridization are region(s) of the genome of the cell to which the protein of binds. A method of identifying a set of genes where cell cycle regulator binding correlates with gene expression and of identifying genomic targets of cell cycle transcription activators in living cells is also encompassed.

US Patent:

20080241822, Oct 2, 2008

Filed:

Dec 21, 2001

Appl. No.:

10/032281

Inventors:

John Wyrick - Pasadena CA, US
Richard A. Young - Weston MA, US
Bing Ren - San Diego CA, US
Francois Robert - Boston MA, US
Itamar Simon - Brighton MA, US

Assignee:

Whitehead Institute for Biomedical Research - Cambridge MA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 912

Abstract:

The present invention relates to a method of identifying a region (one or more) of a genome of a cell to which a protein of interest binds. In the methods described herein, DNA binding protein of a cell is linked (e.g., covalently crosslinked) to genomic DNA of a cell. The genomic DNA to which the DNA binding protein is linked is removed and combined or contacted with DNA comprising a sequence complementary to genomic DNA of the cell under conditions in which hybridization between the identified genomic DNA and the sequence complementary to genomic DNA occurs. Region(s) of hybridization are region(s) of the genome of the cell to which the protein of binds. A method of identifying a set of genes where cell cycle regulator binding correlates with gene expression and of identifying genomic targets of cell cycle transcription activators in living cells is also encompassed.

US Patent:

6410243, Jun 25, 2002

Filed:

Sep 1, 2000

Appl. No.:

09/654409

Inventors:

John Wyrick - Cambridge MA
Richard A. Young - Weston MA
Bing Ren - Somerville MA
Francois Robert - Boston MA

Assignee:

Whitehead Institute for Biomedical Research - Cambridge MA

International Classification:

C12Q 168

US Classification:

435 6, 435 71, 435 78, 435 911, 435 912, 4353201, 435440, 364496, 364497, 364578, 702127, 702 30, 514 12, 514 44, 530350, 536 241

Abstract:

The present invention relates to a method of identifying a region (one or more) of a genome of a cell to which a protein of interest binds. In the methods described herein, DNA binding protein of a cell is linked (e. g. , covalently crosslinked) to genomic DNA of a cell. The genomic DNA to which the DNA binding protein is linked is removed and combined or contacted with DNA comprising a sequence complementary to genomic DNA of the cell under conditions in which hybridization between the identified genomic DNA and the sequence complementary to genomic DNA occurs. Region(s) of hybridization are region(s) of the genome of the cell to which the protein of binds.