John J Szucs

US Patent:

20040166551, Aug 26, 2004

Filed:

Feb 24, 2003

Appl. No.:

10/372745

Inventors:

John Moulds - Pipersville PA, US
Alex Zislin - Princeton Junction NJ, US
John Szucs - Morris Plains NJ, US

International Classification:

C12Q001/56
C12M001/34

US Classification:

435/013000, 435/287100

Abstract:

An apparatus for conducting an assay having an agglutination or size separation step, includes: a first section disposed to receive a fluid to be assayed; and a second section disposed to receive the fluid from the first section upon application of a motive force, preferably centrifugal force, to the fluid, the second section comprising elements fixed to a substrate and adapted to mix the fluid and trap agglutinated particles. In a preferred embodiment, the elements are shaped as pillars. In another preferred embodiment, a third section is provided after the second section and the apparatus is in the form of a disk, preferably an optical disk, having a central axis, and wherein the first, second and third section are arranged in the disk as channels in a direction away from the central axis, respectively. A method for assaying a fluid that has particles to be separated or agglutinated includes: providing a fluid to be assayed into a first section of an apparatus as described above; applying a motive force to the fluid to move the fluid from the first section into a second section, wherein said second section comprises elements fixed to a substrate and adapted to mix the fluid and trap particles in the fluid; and measuring a property of the fluid. In a preferred embodiment, the fluid to be assayed is blood.