John Manuel Salmeron

US Patent:

6346655, Feb 12, 2002

Filed:

Mar 29, 2000

Appl. No.:

09/538414

Inventors:

Thomas M. Hohn - Chapel Hill NC
Cheryl Peters - Raleigh NC
John Salmeron - Hillsborough NC

Assignee:

Syngenta Participations AG - Basel

International Classification:

C12N 1509

US Classification:

800279, 800278, 800288, 800298, 800295, 8003201, 8003203, 536 231, 536 232, 536 237, 435 691, 435419, 435468, 435430, 435177

Abstract:

The present invention discloses trichothecene-resistant transgenic plants, plant tissues, plant seeds, and plant cells comprising a heterologous polynucleotide encoding a gene product having tricothecene resistance activity that thereby confers trichothecene resistance to the transgenic plants, plant tissues, plant seeds, and plant cells. Trichothecene resistance activity, as used herein, refers to an activity that reduces or inhibits the phytotoxicity of a trichothecene, particularly to a fungus and/or plant. In a particular embodiment, trichothecene resistance activity refers to an activity that transfers an acetate to the C-3 position of a trichothecene such as T-2 toxin, HT-2 toxin, isotrichodermol, diacetoxyscirpenol (âDASâ), 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and deoxynivalenol (âDONâ) and their various acetylated derivatives. In another particular embodiment, the gene product having trichthecene resistance activity is a 3-O-acetyltransferase from a trichothecene-producing species of Fusarium, such as or.

US Patent:

6528702, Mar 4, 2003

Filed:

Mar 6, 2000

Appl. No.:

09/519232

Inventors:

John Manuel Salmeron - Hillsborough NC
Laura Jean Weislo - Raleigh NC
Michael G. Willits - Apex NC

Assignee:

Syngenta Participations AG - Basel

International Classification:

A01H 100

US Classification:

800278, 536 231, 536 236, 536 243, 435 701, 4353201, 435410, 435419, 435468, 800295

Abstract:

Homologues of the Arabidopsis NIM1 gene, which is involved in the signal transduction cascade leading to systemic acquired resistance (SAR), are isolated from (tobacco), (tomato), (oilseed rape), (sugarbeet), (sunflower), and (potato). The invention further concerns transformation vectors and processes for expressing the NIM1 homologues in transgenic plants to increase SAR gene expression and enhance broad spectrum disease resistance.

US Patent:

6646184, Nov 11, 2003

Filed:

Feb 12, 2002

Appl. No.:

10/074279

Inventors:

Thomas M. Hohn - Chapel Hill NC
Cheryl Peters - Raleigh NC
John Salmeron - Hillsborough NC

Assignee:

Syngenta Participations AG - Basel

International Classification:

C12N 1509

US Classification:

800279, 800278, 800298, 800295, 8003201, 8003203, 800288, 536 237, 536 232

Abstract:

The present invention discloses trichothecene-resistant transgenic plants, plant tissues, plant seeds, and plant cells comprising a heterologous polynucleotide encoding a gene product having tricothecene resistance activity that thereby confers trichothecene resistance to the transgenic plants, plant tissues, plant seeds, and plant cells. Trichothecene resistance activity, as used herein, refers to an activity that reduces or inhibits the phytotoxicity of a trichothecene, particularly to a fungus and/or plant. In a particular embodiment, trichothecene resistance activity refers to an activity that transfers an acetate to the C-3 position of a trichothecene such as T-2 toxin, HT-2 toxin, isotrichodermol, diacetoxyscirpenol (âDASâ), 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and deoxynivalenol (âDONâ) and their various acetylated derivatives. In another particular embodiment, the gene product having trichthecene resistance activity is a 3-O-acetyltransferase from a trichothecene-producing species of Fusarium, such as or.

US Patent:

6956115, Oct 18, 2005

Filed:

Nov 27, 2002

Appl. No.:

10/305770

Inventors:

Manuel B. Sainz - Durham NC, US
John Salmeron - Hillsborough NC, US

Assignee:

Syngenta Participations AG - Basel

International Classification:

C12N015/29
C12N015/09
A01H005/00

US Classification:

536 236, 4353201, 435 691, 536 231

Abstract:

The present invention pertains to isolated nucleic acid molecules comprising nucleotide sequences isolated from that encode RARI proteins involved in disease resistance, and recombinant vectors comprising said nucleotide sequences.

US Patent:

7049421, May 23, 2006

Filed:

Jul 7, 2003

Appl. No.:

10/614954

Inventors:

Thomas M. Hohn - Chapel Hill NC, US
Cheryl Peters - Raleigh NC, US
John Salmeron - Hillsborough NC, US

Assignee:

Syngenta Participations AG - Schwarzwaldallee

International Classification:

C12N 15/09
C12N 15/31
A01H 5/00

US Classification:

536 232, 536 237, 4353201

Abstract:

The present invention discloses isolated polynucleotides encoding polypeptides having trichothecene 3-O-acetyltransferase activity, recombinant vectors and host cell comprising said polynucleotides.

US Patent:

7098378, Aug 29, 2006

Filed:

Dec 14, 2004

Appl. No.:

11/011906

Inventors:

Manuel B. Sainz - Durham NC, US
John Salmeron - Hillsborough NC, US

Assignee:

Syngenta Participations AG - Basel

International Classification:

C12N 15/09
C12N 15/29
C12N 15/82
A01H 5/00
A01H 5/10

US Classification:

800279, 800278, 800298, 800295, 800317, 8003201, 435 691, 435468

Abstract:

The present invention pertains to transgenic plants comprising nucleic acid molecules comprising nucleotide sequences that encode RAR1 proteins involved in disease resistance, and the RAR1 polypeptides. The invention particularly relates to methods of altering the expressing nucleic acid molecules encoding RAR1 proteins in transgenic plants to alter the level disease resistance, and to transgenic plants, progeny and seed therefrom, having altered enhanced disease resistance. The invention further relates to methods of enhancing expression of R resistance genes, disease resistance signal transduction genes, genes involved in mediating disease resistance or involved in the synthesis of molecules mediating disease resistance. The invention also relates to methods of regulating the expression of other coding sequences of interest by increasing the expression of the nucleic acid molecules of the invention.

US Patent:

7179601, Feb 20, 2007

Filed:

Jul 2, 2003

Appl. No.:

10/613472

Inventors:

Frederick M. Ausubel - Newton MA, US
Brian J. Staskawicz - Castro Valley CA, US
Andrew F. Bent - Madison WI, US
Douglas Dahlbeck - Castro Valley CA, US
Fumiaki Katagiri - San Diego CA, US
Barbara N. Kunkel - St. Louis MO, US
Michael Nicholas Mindrinos - Menlo Park CA, US
Guo-Liang Yu - Berkeley CA, US
Barbara Baker - Berkeley CA, US
Jeffrey Ellis - Weetangera Act 2614, AU
John Salmeron - Hillsborough NC, US

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

The invention relates to a method of identifying a plant disease-resistance gene that includes the steps of (a) providing a plant tissue sample; (b)introducing by biolistic transformation into the plant tissue sample a candidate plant disease-resistance gene; (c) expressing the candidate plant disease-resistance gene within the plant tissue sample; and (d) determining whether the plant tissue sample exhibits a disease-resistance response, whereby a response identifies a plant disease-resistance gene.

US Patent:

20020147324, Oct 10, 2002

Filed:

May 29, 2001

Appl. No.:

09/867852

Inventors:

Frederick Ausubel - Newton MA, US
Brian Staskawicz - Castro Valley CA, US
Andrew Bent - Madison WI, US
Douglas Dahlbeck - Castro Valley CA, US
Fumiaki Katagiri - San Diego CA, US
Barbara Kunkel - St. Louis MO, US
Michael Mindrinos - Menlo Park CA, US
Guo-Liang Yu - Berkeley CA, US
Barbara Baker - Berkeley CA, US
Jeffrey Ellis - Weetangera, AU
John Salmeron - Hillsborough NC, US

International Classification:

C07H021/04
C12N015/00

US Classification:

536/023400, 435/320100

Abstract:

Disclosed is substantially pure DNA encoding an Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.