The present invention provides novel methods for modifying target nucleotide sequences in a plant cell through the use of homologous recombination. The plant cell is transformed with a DNA construct comprising a polynucleotide sequence encoding a fusion polypeptide comprising a polypeptide sequence of interest linked to a reporter sequence.
Methods And Compositions For Independent Dna Replication In Eukaryotic Cells
Edwin F. Ullman - Atherton CA John Jelesko - Seattle WA Marcel R. Pirio - San Jose CA Thomas D. Kempe - Sunnyvale CA
Assignee:
Dade Behring Marburg GmbH - Marburg
International Classification:
C07K 1600 G01N 33536 G01N 33543 G01N 33577
US Classification:
435 77
Abstract:
Receptors are disclosed that are antibodies that exhibit a binding affinity for an immune complex of a monoepitopic antigen and an antibody for such antigen that is substantially greater than the binding affinity for the monoepitopic antigen or the antibody for the monoepitopic antigen apart from the immune complex. Normally, the monoepitopic antigen has a molecular weight less than 1500 and is an organic compound. The antibodies of the present invention find use in a method for determining a monoepitopic antigen in a sample suspected of containing such antigen. The method comprises forming an immune sandwich complex comprising the monoepitopic antigen or an analog thereof, a first monoclonal antibody that binds to the monoepitopic antigen, and a second monoclonal antibody that is an antibody of the present invention and detecting the immune sandwich complex. Compositions of matter and kits for use in conducting an assay in accordance with the invention as well as methods for producing the above receptors are also disclosed.
Edwin F. Ullman - Atherton CA John Jelesko - Seattle WA Marcel R. Pirio - San Jose CA Thomas D. Kempe - Sunnyvale CA
Assignee:
Syntex (U.S.A.) Inc. - Palo Alto CA
International Classification:
C07K 1528 G01N 33577
US Classification:
436547
Abstract:
Receptors are disclosed that are antibodies that exhibit a binding affinity for an immune complex of a monoepitopic antigen and an antibody for such antigen that is substantially greater than the binding affinity for the monoepitopic antigen or the antibody for the monoepitopic antigen apart from the immune complex. In one embodiment the receptors are antibodies prepared against an affinity-labeled complex of an antigen and an antibody for the antigen. Normally, the monoepitopic antigen has a molecular weight less than 1500 and is an organic compound. The antibodies of the present invention find use in a method for determining a monoepitopic antigen in a sample suspected of containing such antigen. The method comprises forming an immune sandwich complex comprising the monoepitopic antigen or an analog thereof, a first monoclonal antibody that binds to the monoepitopic antigen, and a second monoclonal antibody that is an antibody of the present invention and detecting the immune sandwich complex. Compositions of matter and kits for use in conducting an assay in accordance with the invention as well as methods for producing the above receptors are also disclosed.
Compositions And Methods Comprising Colletotrichum For Controlling Plant Species
John G. Jelesko - Blacksburg VA, US Matthew Kasson - Christiansburg VA, US
Assignee:
VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. - Blacksburg VA
International Classification:
A01N 63/04
US Classification:
504117
Abstract:
Provided are methods and compositions for controlling at least one plant. Methods and compositions can comprise at least one strain of spp., such as TR-123, TR-126, and combinations thereof. Methods and compositions of the invention are useful, for example, for controlling and/or