John W Dame

US Patent:

47074450, Nov 17, 1987

Filed:

Jul 31, 1984

Appl. No.:

6/636261

Inventors:

Thomas F. McCutchan - Silver Spring MD
John B. Dame - Rockville MD

Assignee:

The United States of America as represented by the Secretary of the
Department of Health and Human Services - Washington DC

International Classification:

C12P 1934
C12N 1500
C07H 1512

US Classification:

435 91

Abstract:

The present invention discloses isolated functionally intact whole genes and method of obtaining the same. The method includes treating genomic DNA with mung bean nuclease and formamide under controlled conditions. The invention also discloses cloning of said intact whole genes and a library of such cloned genes or any recombinations thereof. The invention is useful in deriving gene products as m-RNA, S-RNA, t-RNA, polypeptides and the like.

US Patent:

51224712, Jun 16, 1992

Filed:

Feb 9, 1989

Appl. No.:

7/308219

Inventors:

Mark C. Jenkins - Bowie MD
Hyun S. Lillehoj - West Friendship MD
John B. Dame - Gainesville FL
Harry D. Danforth - Severn MD
Michael D. Ruff - Bowie MD

Assignee:

The United States of America as represented by the Secretary of
Agriculture - Washington DC

International Classification:

C12N 121
C12N 1500
C12N 1570
C12P 2102
C12P 1934

US Classification:

4352523

Abstract:

Disclosed are DnA sequences which code for antigenic proteins, methods for identifying such DNA sequences, and antigens coded for by such DNA sequences. The first step of the method is to provide a multiplicity of DNA sequences. These sequences are then inserted into DNA expression vectors to form recombinant expression vectors. The expression vectors are inserted into suitable hosts to form transformants which express the DNA sequences. The transformants are then contacted with antibodies directed against Eimeria antigens to identify transformants containing DNA sequences which code for Eimeria antigens. These antigens are then produced from the DNA sequences identified as coding for the antigens. The antigens so produced are contacted with white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein, to thereby identify DNA sequences which code for antigens that induce a cell-mediated immune response to avian coccidiosis. The DNA sequences of the present invention comprise cloned genes or fragments thereof that code on expression for an antigenic protein that activates white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein.