John E Arruda

US Patent:

58009949, Sep 1, 1998

Filed:

Jul 24, 1996

Appl. No.:

8/685793

Inventors:

Richard A. Martinelli - Brighton MA
John C. Arruda - Attleboro MA

Assignee:

Chiron Diagnostics Corporation - East Walpole MA

International Classification:

C12Q 168
C12Q 170
C12P 1934
C07H 2104

US Classification:

435 6

Abstract:

A new method has been developed for conducting a gene probe assay. The preferred technique involves (1) using a gene amplification technique (e. g. , PCR) to multiply the gene sequence of interest and (2) using a hybridization--ligation detection methodology, wherein the sequences of probes hybridized to the target sequence allow for separation and detection (e. g. , probes might contain a combination of magnetic particles and acridinium esters) to determine if a specific sequence is present.