Jerry H Meldon

US Patent:

45334471, Aug 6, 1985

Filed:

Jun 13, 1983

Appl. No.:

6/503916

Inventors:

Jerry H. Meldon - Brookline MA

International Classification:

B01D 1706
G01N 2726
G01N 2728

US Classification:

2041814

Abstract:

An apparatus for and a method for separating a mixture of proteins containing proteins of different isoelectric points, which method comprises flowing an aqueous feed stream containing the mixture of proteins along a thin-channel flow path established between first and second gas-permeable membranes, diffusing a gas through the first and second membranes and generally transversely across the flow path of the feed stream, the gas selected to establish a gradient in the concentration of the gas and to provide a gradient in pH across the flow path of the feed stream, and, after a defined time period, separating the feed stream into streams containing concentrated fractions of proteins having defined isoelectric points, by virtue of the difference in the direction of diffusion occurring of the proteins in the flow path.