Jenna L Balestrini

US Patent:

20200115668, Apr 16, 2020

Filed:

Dec 11, 2019

Appl. No.:

16/711284

Inventors:

- Cambridge MA, US
Jenna L. Balestrini - Boston MA, US
Vishal Tandon - Roxbury Crossing MA, US
Jonathan R. Coppeta - Windham NH, US

International Classification:

C12M 1/42
C12M 1/12
B01L 3/00
C12M 3/06
C12M 1/00
C12N 13/00
C12M 1/34

Abstract:

A system and method of using a microfluidic electroporation device for cell treatment is provided. The cell or exosome treatment system can include a microfluidic electroporation device, a voltage source coupled to a plurality of electrodes and a controller coupled to the voltage source. The microfluidic electroporation device can include a fluid receptacle, a semipermeable membrane, and a base including a channel in fluid communication with the fluid receptacle and the semipermeable membrane. A first electrode can be positioned within the fluid receptacle and a second electrode coupled to the base. The second electrode is positioned relative to the first electrode to create an electric field sufficient to electroporate cells or exosomes disposed in the fluid receptacle. The controller can be configured to cause the first and second electrodes to apply voltage electroporating the cells and exosomes.

US Patent:

20200071727, Mar 5, 2020

Filed:

Aug 30, 2019

Appl. No.:

16/557820

Inventors:

- Cambridge MA, US
Charles A. Lissandrello - Newtonville MA, US
Jenna L. Balestrini - Boston MA, US
Jonathan R. Coppeta - Windham NH, US
Patricia A. Swierk - Cambridge MA, US

International Classification:

C12N 15/87
C12Q 1/02
C12M 1/42
B01L 3/00

Abstract:

Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.

US Patent:

20190292510, Sep 26, 2019

Filed:

Mar 20, 2019

Appl. No.:

16/359629

Inventors:

- Cambridge MA, US
Jonathan R. Coppeta - Windham NH, US
Kenneth Kotz - Newton MA, US
Heena K. Mutha - Cambridge MA, US
Jenna Leigh Balestrini - Boston MA, US

International Classification:

C12M 1/42
C12N 13/00
B01L 3/00
C12M 1/34
C12N 15/87

Abstract:

A viral transduction and/or electroporation device has s a membrane separating two chambers and two electroporation electrodes for the chambers. An electrical voltage source is used for establishing an electrical field across the membrane and between the two electrodes. In operation, fluid is flowed into the chambers including fluid containing electroporation cargo and viral transduction solution and an electrical field is established across the membrane and between the electrodes to electroporate cells pinned to the membrane and transfecting the cells.

US Patent:

20190292565, Sep 26, 2019

Filed:

Mar 20, 2019

Appl. No.:

16/359626

Inventors:

- Cambridge MA, US
Charles A. Lissandrello - Newtonville MA, US
Jenna Leigh Balestrini - Boston MA, US
Ryan A. Dubay - Ludlow MA, US

International Classification:

C12N 15/87
C12N 13/00
C12N 15/85
C12M 1/42

Abstract:

A system for sequential exposure of particles to different fluid streams includes an acoustic actuator device for acoustically driving one or more substrates and a microchannel device of the one or more substrates that receive particles in a first flowing fluid, moves the particles to a second flowing fluid, then moves the particles out of the second flowing fluid using acoustic radiation generated by the acoustic actuator device. The system can control residence times in the streams. According to one use, the first flowing fluid is a cell buffer and the second flowing media is an electroporation buffer. An electroporation system is placed in or downstream of the acoustic actuator device. However, in other uses, the second flowing media might be a wash buffer.

US Patent:

20190071628, Mar 7, 2019

Filed:

Sep 6, 2018

Appl. No.:

16/123539

Inventors:

- Cambridge MA, US
Jenna L. Balestrini - Boston MA, US
Vishal Tandon - Roxbury Crossing MA, US
Louis B. Kratchman - Quincy MA, US

International Classification:

C12M 1/42
C12N 13/00
C12N 15/87

Abstract:

Microfluidic devices and associated methods are disclosed. A microfluidic device includes a target entrainment channel and an effluent channel on opposing sides of a semipermeable membrane. A restrictor channel that is narrower than the effluent channel is interposed between the semipermeable membrane and the effluent channel. Fluid that flows from the target entrainment channel, through the semipermeable membrane and the restrictor channel to the effluent channel, pins target cells along the center of the target entrainment channel for electroporation using an electrode in the channel.

US Patent:

20190010516, Jan 10, 2019

Filed:

Jul 3, 2018

Appl. No.:

16/026709

Inventors:

- Cambridge MA, US
Daniel K. Freeman - Cambridge MA, US
Jonathan R. Coppeta - Windham NH, US
Jeffrey T. Borenstein - Newton MA, US
Jenna L. Balestrini - Boston MA, US

International Classification:

C12N 15/87

Abstract:

A method and system of delivering a charged cargo, such as a biomolecule, to a target structure, such as cells, exosomes, other vesicles or micelles, using an electroactive porous membrane. This method comprises contacting an electroactive porous membrane with a fluid flow toward the membrane. The fluid contains charged biomolecules and the membrane and biomolecules are oppositely charged so that the biomolecules in the fluid are trapped on the membrane as the fluid flows through the pores of the membrane. Acceptor cells of interest are pinned to the membrane by the flow of the fluid, thereby aggregating the cells onto the membrane in close proximity to the trapped biomolecules. Finally, the acceptor cells are permeabilized.

US Patent:

20180362908, Dec 20, 2018

Filed:

Aug 22, 2018

Appl. No.:

16/109089

Inventors:

- Cambridge MA, US
Joseph L. Charest - Cambridge MA, US
Christopher M. DiBiasio - Stoughton MA, US
Dorit Berlin - Lexington MA, US
Jenna Balestrini - Boston MA, US
Jose A. Santos - Westwood MA, US
Vishal Tandon - Roxbury Crossing MA, US
Jason O. Fiering - Boston MA, US

International Classification:

C12M 3/06
C12M 1/00
B01L 3/00
C12M 1/26
C12M 1/12
G01N 1/34
C12N 15/86
C12N 15/87
C12N 5/0783
C12M 1/42

Abstract:

Systems and methods are disclosed herein for use in transducing, activating, and otherwise treating cells. Cells are introduced into an inner layer of a multi-layered stack that defines at least one flow chamber and a plurality of cell entrainment regions. Vertical flow through the stack entrains the cells in the cell entrainment regions along with genetic information introduction agents or other additives, before the cells are washed using a reverse vertical flow and are collected from the device.

US Patent:

20180179485, Jun 28, 2018

Filed:

Dec 21, 2017

Appl. No.:

15/851393

Inventors:

- Cambridge MA, US
Jenna L. Balestrini - Boston MA, US
Vishal Tandon - Roxbury Crossing MA, US
Jonathan R. Coppeta - Windham NH, US

International Classification:

C12M 1/42
C12N 15/87
C12N 13/00
C12M 1/12
C12M 1/00
C12M 3/06
B01L 3/00

Abstract:

A system and method of using a microfluidic electroporation device for cell treatment is provided. The cell or exosome treatment system can include a microfluidic electroporation device, a voltage source coupled to a plurality of electrodes and a controller coupled to the voltage source. The microfluidic electroporation device can include a fluid receptacle, a semipermeable membrane, and a base including a channel in fluid communication with the fluid receptacle and the semipermeable membrane. A first electrode can be positioned within the fluid receptacle and a second electrode coupled to the base. The second electrode is positioned relative to the first electrode to create an electric field sufficient to electroporate cells or exosomes disposed in the fluid receptacle. The controller can be configured to cause the first and second electrodes to apply voltage electroporating the cells and exosomes.