Jekwan Ryu

US Patent:

20050239113, Oct 27, 2005

Filed:

Apr 6, 2005

Appl. No.:

11/101019

Inventors:

Jekwan Ryu - Cupertino CA, US
Richard Rava - Redwood City CA, US
Stephen Fodor - Palo Alto CA, US

Assignee:

Affymetrix, INC. - Santa Clara CA

International Classification:

C12Q001/68
G06F019/00
G01N033/48
G01N033/50

US Classification:

435006000, 702020000

Abstract:

The present invention provides methods and devices for high sensitivity and high speed microarray optical imaging. The methods include using patterned excitation to obtain a series of images and analyzing the images to resolve probe intensities which reflect the hybridization or binding between target and probes. Probe feature information and patterned excitation (structured illumination) information are incorporated into the analysis.

US Patent:

20050239114, Oct 27, 2005

Filed:

Apr 6, 2005

Appl. No.:

11/101043

Inventors:

Jekwan Ryu - Cupertino CA, US
Richard Rava - Redwood City CA, US
Stephen Fodor - Palo Alto CA, US

Assignee:

Affymetrix, INC. - Santa Clara CA

International Classification:

C12Q001/68
G06F019/00
G01N033/48
G01N033/50

US Classification:

435006000, 702020000

Abstract:

The present invention provides methods and devices for high sensitivity and high speed microarray optical imaging. The methods include using patterned excitation to obtain a series of images and analyzing the images to resolve probe intensities which reflect the hybridization or binding between target and probes. Probe feature information and patterned excitation (structured illumination) information are incorporated into the analysis.

US Patent:

20050239115, Oct 27, 2005

Filed:

Apr 6, 2005

Appl. No.:

11/101087

Inventors:

Jekwan Ryu - Cupertino CA, US
Richard Rava - Redwood City CA, US
Stephen Fodor - Palo Alto CA, US

Assignee:

Affymetrix, INC. - Santa Clara CA

International Classification:

C12Q001/68
G06F019/00
G01N033/48
G01N033/50

US Classification:

435006000, 702020000

Abstract:

The present invention provides methods and devices for high sensitivity and high speed microarray optical imaging. The methods include using patterned excitation to obtain a series of images and analyzing the images to resolve probe intensities which reflect the hybridization or binding between target and probes. Probe feature information and patterned excitation (structured illumination) information are incorporated into the analysis.

US Patent:

20090061505, Mar 5, 2009

Filed:

Aug 28, 2007

Appl. No.:

11/846067

Inventors:

Stanley S. Hong - Palo Alto CA, US
Jekwan Ryu - Cupertino CA, US
Jong Buhm Park - Sunnyvale CA, US

International Classification:

C12M 1/00

US Classification:

4352872

Abstract:

Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an optical fiber delivering a first laser beam, and an interference pattern generation module coupled to the optical fiber. The interference pattern generation module splits the first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams.

US Patent:

20090061526, Mar 5, 2009

Filed:

Aug 28, 2007

Appl. No.:

11/846049

Inventors:

Stanley S. Hong - Palo Alto CA, US
Jekwan Ryu - Cupertino CA, US
Jong Buhm Park - Sunnyvale CA, US

International Classification:

G01N 33/00
G06K 9/74

US Classification:

436 94, 382129

Abstract:

Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more reagents, selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an optical fiber delivering a first laser beam, and an interference pattern generation module coupled to the optical fiber. The interference pattern generation module splits the first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams.

US Patent:

20140323325, Oct 30, 2014

Filed:

Mar 4, 2014

Appl. No.:

13/999508

Inventors:

Marc Beal - Concord CA, US
Ronald M. Cook - Novato CA, US
Peter Jay Coassin - Encinitas CA, US
Arturo Orjalo - Oakland CA, US
Jekwan Ryu - Cupertino CA, US

International Classification:

C12Q 1/68
G01N 21/64

US Classification:

506 9, 436501, 435 611, 435 5

Abstract:

The present invention generally relates to imaging single molecules, or one or more collections of single molecules, and methods related to the imaging. In a method aspect, the present invention provides a method of imaging single molecules. The method comprises the steps of: a) exposing a test sample to a probe, wherein the probe comprises a first portion that specifically binds to a target molecule and a second portion that is detectable as the result of one or more chemical groups that interact with light at one or more wavelengths, wherein the probe binds to a target molecule to provide a complex; b) exposing the complex to one or more wavelengths of light that interact with the one or more chemical groups; c) detecting a result from the interacting of one or more wavelengths of light that interact with the one or more chemical groups to provide an image of one or more single molecules. The image possesses a resolution better than 450 nm over a view field area of at least 1×10μm, and wherein the image is obtained in a single detection step without variation of any detection settings.

US Patent:

20140242683, Aug 28, 2014

Filed:

May 5, 2014

Appl. No.:

14/270134

Inventors:

- Santa Clara CA, US
Jekwan Ryu - Cupertino CA, US
Jong Buhm Park - Sunnyvale CA, US

Assignee:

LightSpeed Genomics, Inc. - Santa Clara CA

International Classification:

C12Q 1/68

US Classification:

4352872

Abstract:

Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more reagents, selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an interference pattern generation module that splits a first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams.