Jeffrey S Biddle

US Patent:

20190048312, Feb 14, 2019

Filed:

Jul 13, 2018

Appl. No.:

16/035344

Inventors:

Christine M. Biddle - Northbrook IL, US
Jeffrey W. Biddle - Buffalo NY, US
Laurel Biddle - Buffalo NY, US
- Carlsbad CA, US
Barbara Dadey - East Aurora NY, US

International Classification:

C12N 5/00
C12N 1/00

Abstract:

The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.