Jay J. Thelen - Columbia MO, US Severin E. Stevenson - Columbia MO, US
International Classification:
C40B 30/10 C40B 60/12
US Classification:
506 12, 506 39
Abstract:
The invention provides a high-throughput method for quantitating plant seed proteins, e.g. seed allergens. Such method involves obtaining a protein sample from the seed; analyzing the sample using mass spectrometry with a set of pre-designed internal standard peptides that are specific to the plant allergens; and monitoring frequencies and intensities of resulting spectra to obtain relative and absolute allergen contents in the seed. The invention also provides a system for high-throughput profiling of plant seed allergens. Such system comprises a set of pre-designed internal standard peptides that are specific to the plant seed allergens and one or more mass spectrometers.
Pyruvate Dehydrogenase Kinase Polynucleotides, Polypeptides And Uses Thereof
Douglas D. Randall - Columbia MO Jay J. Thelen - Columbia MO Jan A. Miernyk - Peoria IL Michael G. Muszynski - Des Moines IA Vincent J. H. Sewalt - West Des Moines IA
Assignee:
Pioneer Hi-Bred International, Inc. - Des Moines IA University of Missouri - Columbia MO
International Classification:
C12N 504 C12N 1529 C12N 1582 A01H 500 A01H 510
US Classification:
800284
Abstract:
The invention provides methods and compositions relating to altering carbohydrate metabolism and/or composition of plants. The invention provides isolated nucleic acids and their encoded proteins, expression cassettes, host cells, transgenic plants, and antibody compositions.
Increasing Plant Oil Content By Improving Activity Of Acetyl-Coa Carboxylase
The Curators of the University of Missouri - Columbia MO
International Classification:
C12N 15/82 C12N 9/12 C12N 15/52
Abstract:
The present invention provides a method and means to change fatty acid and ultimately triacylglycerol production in plants and algae. Methods of the invention comprise the step of altering the activity levels of the committed step for de novo fatty acid biosynthesis, acetyl-CoA carboxylases (ACCase). More specifically, methods of the invention directly enhance the activity of ACCase by overexpression of α-CT or a catalytic portion thereof.
University of Missouri Sep 2004 - Sep 2016
Associate Professor
University of Missouri Sep 2004 - Sep 2016
Professor
University of Missouri Sep 2004 - Sep 2009
Assistant Professor
University of Missouri Jun 2002 - Aug 2004
Associate Director of Proteomics Center
Michigan State University 1999 - Jun 2002
Postdoctoral Scientist
Education:
University of Missouri - Columbia 1994 - 1998
Doctorates, Doctor of Philosophy, Biology, Biochemistry
University of Nebraska - Lincoln 1989 - 1993
Bachelors, Bachelor of Science, Biology, Biochemistry
Skills:
Molecular Biology Biochemistry Protein Chemistry Proteomics Bioinformatics Life Sciences Science Genomics Genetics Pcr Mass Spectrometry Computational Biology Protein Purification Western Blotting Microbiology Sds Page Gel Electrophoresis Protein Expression Chromatography Lifesciences Lc Ms Dna Purification Systems Biology Immunoprecipitation