2004 to Jun 2013 Director, Transgenic and GeneMouse Genetics and Genome Philadelphia, PA 1999 to 2004 DirectorTransgenic and Gene Knockout Facility Philadelphia, PA 1995 to 1999 Biochemistry and Molecular Pharmacology and DirectorLaboratory of Mol Genetics, NIH Bethesda, MD 1985 to 1987 Fogarty AssociateLaboratory of Mole. Genetics, NIH Bethesda, MD 1981 to 1984 Fogarty Fellow
Education:
Punjab University 1980 Ph.D. in BiochemistryPunjab University 1974 M.Sc. in BiochemistryPunjab University 1972 B.Sc. in Biology/Chemistry
Us Patents
Transgenic Col2A1-Null Mice Expressing Human Col2A1
Transgenic animals which express human collagen gene and have an inactivated endogenous collagen gene are provided. Compositions comprising humanized chondrocytes isolated from these transgenic animals are also provided. In addition, methods of screening potential therapeutics for cartilage related disorders in these animals are provided.
Method For Programmed Differentiation Of Stem Cells
Provided is a method for programming the differentiation of stem cells and for the identification of the signals responsible for cell lineage establishment of differentiated cells.
Use Of A Col1A1 Mini-Gene Construct To Inhibit Collagen Synthesis
Darwin J. Prockop - Philadelphia PA Jaspal Khillan - Cherry Hill NJ Ruth Pereira - Philadelphia PA
Assignee:
Thomas Jefferson University - Philadelphia PA
International Classification:
A61K 4800 C07H 2104 C12N 1512 C12N 1585
US Classification:
514 44
Abstract:
Methods are provided for controlling the synthesis of protein from the human gene for the pro. alpha. 1(I) chain of type I procollagen in cells using mini-gene constructs which are injected into cells which synthesize the protein. Methods for controlling collagen deposition in tissue mini-gene constructs are also provided.
Apparatus And Method For Culturing Embryonic Stem Cells
Apparatus and method for culturing at least two different cell types in close proximity are provided. A solid support having at least one depression wherein a controlled concentration of a selected cell type can be layered is employed in the invention.
Efficient Method For Production Of Compound Transgenic Animals