Jason H Bielas

US Patent:

20130288237, Oct 31, 2013

Filed:

Oct 19, 2012

Appl. No.:

13/656265

Inventors:

ADAPTIVE BIOTECHNOLOGIES CORPORATION - , US
FRED HUTCHINSON CANCER RESEARCH CEN - , US
Jason H. Bielas - Seattle WA, US

Assignee:

FRED HUTCHINSON CANCER RESEARCH CENTER - Seattle WA
ADAPTIVE BIOTECHNOLOGIES CORPORATION - Seattle WA

International Classification:

C12Q 1/68

US Classification:

435 611, 435 612

Abstract:

Compositions and methods are described for highly sensitive quantification of the relative representation of DNA from adaptive immune cells (e.g., T and/or B lymphocytes) in DNA extracted from complex mixtures of cells that include cells which are not adaptive immune cells. Included are methods for determining the relative presence in a tumor of tumor infiltrating lymphocytes (TIL), the relative presence of lymphocytes infiltrating a somatic tissue that is the target of an autoimmune disease, and the relative presence of lymphocytes infiltrating a transplanted organ.

US Patent:

20220349004, Nov 3, 2022

Filed:

Jun 29, 2022

Appl. No.:

17/853716

Inventors:

- Seattle WA, US
Jason H. BIELAS - Seattle WA, US

International Classification:

C12Q 1/6874
C12Q 1/6869
C12N 15/10
C12N 15/70
C12N 15/81
C12N 15/85
C40B 40/08
C40B 50/06
C12Q 1/6827

Abstract:

The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

US Patent:

20210395818, Dec 23, 2021

Filed:

Sep 1, 2021

Appl. No.:

17/464341

Inventors:

- Seattle WA, US
Jason H. BIELAS - Seattle WA, US

International Classification:

C12Q 1/6874
C12Q 1/6869
C12N 15/10
C12N 15/70
C12N 15/81
C12N 15/85
C40B 40/08
C40B 50/06
C12Q 1/6827

Abstract:

The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

US Patent:

20210388435, Dec 16, 2021

Filed:

Aug 25, 2021

Appl. No.:

17/412105

Inventors:

- Seattle WA, US
Jason H. BIELAS - Seattle WA, US

International Classification:

C12Q 1/6874
C12Q 1/6869
C12N 15/10
C12N 15/70
C12N 15/81
C12N 15/85
C40B 40/08
C40B 50/06
C12Q 1/6827

Abstract:

The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

US Patent:

20210340619, Nov 4, 2021

Filed:

Jul 13, 2021

Appl. No.:

17/374917

Inventors:

- Seattle WA, US
Jason H. BIELAS - Seattle WA, US

International Classification:

C12Q 1/6874
C12Q 1/6869
C12N 15/10
C12N 15/70
C12N 15/81
C12N 15/85
C40B 40/08
C40B 50/06
C12Q 1/6827

Abstract:

The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

US Patent:

20210317525, Oct 14, 2021

Filed:

Jun 23, 2021

Appl. No.:

17/356288

Inventors:

- Seattle WA, US
Jason H. BIELAS - Seattle WA, US

International Classification:

C12Q 1/6874
C40B 40/08
C40B 50/06
C12N 15/10
C12Q 1/6827
C12N 15/81
C12N 15/85
C12N 15/70
C12Q 1/6869

Abstract:

The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

US Patent:

20210317526, Oct 14, 2021

Filed:

Jun 23, 2021

Appl. No.:

17/356293

Inventors:

- Seattle WA, US
Jason H. BIELAS - Seattle WA, US

International Classification:

C12Q 1/6874
C40B 40/08
C40B 50/06
C12Q 1/6827
C12N 15/10
C12N 15/81
C12N 15/85
C12N 15/70
C12Q 1/6869

Abstract:

The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

US Patent:

20210246504, Aug 12, 2021

Filed:

Apr 20, 2021

Appl. No.:

17/235837

Inventors:

- Seattle WA, US
Jason H. BIELAS - Seattle WA, US

International Classification:

C12Q 1/6874
C12Q 1/6869
C12N 15/10
C12N 15/70
C12N 15/81
C12N 15/85
C40B 40/08
C40B 50/06
C12Q 1/6827

Abstract:

The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.