Bruce SELIGMANN - Tucson AZ, US Ralph Martel - Tucson AZ, US Matthew Rounseville - Tucson AZ, US Ihab Botros - Tucson AZ, US
International Classification:
C12Q 1/68
US Classification:
435 6
Abstract:
The present invention relates to compositions, apparatus and methods useful for concurrently performing singular, multiple, high throughput, biological or chemical assays, using nuclease protection molecules which specifically bind to a target of interest. The nuclease protection molecules are capable of detecting targets in complex biological samples, including, preserved, fixed, dried, and/or cross-linked specimen. The reagents and methods of the instant invention provide an effective means for analyzing a target of interest from a complex biological sample without solubilizing or disrupting the sample. Utilization of such methods in clinical and/or diagnostic applications is also described.
- Tucson AZ, US BJ Kerns - Tucson AZ, US John Luecke - Tucson AZ, US Matt Rounseville - Tucson AZ, US Ihab Botros - Tucson AZ, US Mark Schwartz - Tucson AZ, US
Assignee:
HTG Molecular Diagnostics, Inc. - Tucson AZ
International Classification:
C12Q 1/6813 C12Q 1/6827 C12Q 1/6886
Abstract:
Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.
Methods Of Co-Detecting Mrna And Small Non-Coding Rna
- Tucson AZ, US Matt Rounseville - Tucson AZ, US Krishna Maddula - Tucson AZ, US Ihab Botros - Tucson AZ, US Chris Cox - Tucson AZ, US
International Classification:
C12Q 1/68
US Classification:
506 9
Abstract:
Disclosed herein are methods of co-detecting presence of target messenger RNA (mRNA) and small non-coding RNA (for example, miRNA) in a sample. The disclosed methods can be used to simultaneously detect mRNA and small non-coding RNA in a single assay (for example in the same reaction or the same well of a multi-well assay). The methods can include contacting a sample with a plurality of nuclease protection probes including at least one probe which specifically binds to a target mRNA and at least one probe which specifically binds to a target small non-coding RNA, contacting the sample with a nuclease specific for single-stranded nucleic acids, and detecting the NPP, for example on a microarray.
Bruce Seligmann - Tucson AZ, US Bj Kerns - Tucson AZ, US John Luecke - Tucson AZ, US Matt Rounseville - Tucson AZ, US Ihab Botros - Tucson AZ, US Mark Schwartz - Tucson AZ, US
Assignee:
HTG MOLECULAR DIAGNOSTICS, INC. - Tucson AZ
International Classification:
C12Q 1/68
US Classification:
435 611
Abstract:
Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes, and detecting the fusion probe after nuclease treatment. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes, and detecting these probes after nuclease treatment. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.
HTG Molecular Diagnostics - Tucson, Arizona Area since Jan 2001
Scientific Fellow III
Systems Integration Drug Discovery Company (SIDDCO) - Tucson, Arizona Area May 1998 - Jan 2001
Scientific Fellow
Education:
San Diego State University-California State University 1986 - 1989
MS, Molecular Biology
University of California, Los Angeles 1979 - 1984
BS, Microbiology
Skills:
Molecular Biology Biochemistry Biotechnology Genomics Lifesciences Pcr Cell Culture Protein Purification Cell Biology Microbiology Cell Based Assays Elisa Western Blotting Protein Chemistry Life Sciences Cell Immunohistochemistry Dna Sequencing Sequencing Protein Expression Molecular Cloning Immunoassays High Throughput Screening Rna Isolation Molecular Diagnostics Purification Rt Pcr Sds Page Tissue Culture In Vitro Gel Electrophoresis Assay Development Data Analysis Troubleshooting Research Design Process Management Training Customer Support Transfection Proteomics Antibodies Microarray Electrophoresis Dna