Holly C Dippold

Holly Dippold San Diego, CA

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Work:

Department of Medicine, University of California

2009 to 2000
Postdoctoral Fellow University of California
San Diego, CA
2002 to 2009
Graduate Student Researcher, Division of Biology University of Washington
Seattle, WA
2001 to Present
Summer undergraduate research intern

Education:

University of California
San Diego, CA
2002 to 2009
Doctor of Philosophy in Biological Sciences California State University
1999 to 2002
Bachelor of Science in General Biology UCSD Extension Online Course
2006
Additional Coursework in Medical Writing: Protocols, Reports, Summaries, and Submissions

Skills:

Mammalian cell culture (HeLa, HEK293, MCF10A, U2-OS, NIH3T3, CHO, etc.) siRNA Transient expression of exogenous genes in mammalian cells and yeast Immunofluorescence microscopy of fixed mammalian cells Fluorescence microscopy of live mammalian cells and yeast Immunoprecipitation of endogenous and epitope-tagged proteins from yeast and mammalian cell lysates Expression of recombinant proteins in bacteria Affinity purification of recombinant proteins Western blot ELISA Molecular biology techniques including cloning of genes from yeast genomic DNA and mammalian cDNA, primer design, PCR, restriction digest, DNA purification, site-directed mutagenesis, sequence alignment Yeast genetics, including chromosomal deletions and epitope tagging and dissection and analysis of yeast tetrads Protein characterization techniques including subcellular fractionation of organelles by differential centrifugation, determination of size and nature of protein complexes by ultracentrifugation on glycerol velocity and sucrose density gradients, and size exclusion chromatography