Henry G Heinsohn

US Patent:

20110262946, Oct 27, 2011

Filed:

Apr 14, 2011

Appl. No.:

13/087075

Inventors:

Sushmita Mimi Roy - Sunnyvale CA, US
Sunil Bajad - Fremont CA, US
Evan Green - Mountain View CA, US
Henry Heinsohn - Alameda CA, US

Assignee:

Sutro Biopharma, Inc. - South San Francisco CA

International Classification:

C12Q 1/37
C12P 21/00

US Classification:

435 23, 435 681

Abstract:

The present invention provides a method for monitoring of profile changes of components in a dynamic system such as a cell-free in vitro protein synthesis system by using liquid chromatography (LC) combined with mass spectrometry (MS). In an additional aspect, this invention provides a method for enhancing the yield and/or reproducibility in a cell-free protein synthesis system by modulating the level and/or activity of a protein component that has regulatory effects on the system.

US Patent:

53786211, Jan 3, 1995

Filed:

May 7, 1993

Appl. No.:

8/057851

Inventors:

Virgil B. Lawlis - San Mateo CA
Henry G. Heinsohn - Pacifica CA
Enrique F. Baliu - San Bruno CA

Assignee:

Genencor, Inc. - San Francisco CA

International Classification:

C12N 900
C12P 2100

US Classification:

435183

Abstract:

This invention provides a method for killing fungal cells without lysing in fermentation processes in order to prepare the fermentation mixture for processing to recover or extract an extracellularly expressed enzyme from the fermentation mixture. A preferred method of this invention comprises adjusting the pH of the fermentation mixture to less than 2. 79 using a mineral acid, then adding sufficient acetic acid to the mixture to affect a substantially complete cell kill in mixture. A salt of the acetic acid can be used. The organic acid or salt can be added, then the pH adjusted to the desired level. Other organic acids can be used, in which case the pH of the mixture is adjusted to the pK. sub. a of the selected organic acid before the organic acid is added to the mixture. The method of this invention is useful for stopping the growth and killing the cells in any micro-organism, culture or fermentation such as those containing yeast, bacteria or fungi and is particularly useful in systems where it is desired to kill the cells without lysing them.

US Patent:

51513587, Sep 29, 1992

Filed:

Jun 13, 1990

Appl. No.:

7/537461

Inventors:

Henry G. Heinsohn - Pacifica CA
Jeffrey D. Lorch - San Mateo CA
Kirk J. Hayenga - San Mateo CA
Raymond E. Arnold - San Francisco CA

Assignee:

Genencor International, Inc. - South San Francisco CA

International Classification:

C12N 964

US Classification:

435226

Abstract:

Disclosed are methods for the recovery and purification of naturally produced chymosin. In particular, disclosed are methods for the recovery and purification of chymosin from aqueous solutions containing chymosin, pepsin and other contaminants.

US Patent:

58010343, Sep 1, 1998

Filed:

Dec 14, 1994

Appl. No.:

8/356042

Inventors:

Virgil B. Lawlis - San Mateo CA
Henry G. Heinsohn - Pacifica CA
Enrique F. Baliu - San Francisco CA

Assignee:

Genencor International - Rochester NY

International Classification:

C12N 900
C12P 2100

US Classification:

435183

Abstract:

This invention provides a method for killing cells in fermentation mixtures in order to prepare the fermentation mixture for processing to recover or extract a desired product from the fermentation mixture. A preferred method of this invention comprises in either order, adjusting the pH of the fermentation mixture to a value equal to or less than about two pH units below the pK. sub. a of the compatible organic acid using a mineral acid, and adding a sufficient amount of a compatible organic acid and/or organic acid salt to the mixture to effect a substantially complete cell kill. The method of this invention is useful for killing microorganisms such as yeast, bacteria or fungi in any culture or fermentation mixture and is particularly useful in systems where it is desired to kill the cells without lysing them.

US Patent:

52159086, Jun 1, 1993

Filed:

Apr 16, 1992

Appl. No.:

7/869838

Inventors:

Henry G. Heinsohn - Pacifica CA
Matthew B. Murphy - San Francisco CA

Assignee:

Genencor International, Inc. - Rochester NY

International Classification:

C12N 964

US Classification:

435226

Abstract:

This invention provides a method of recovering and purifying chymosin from fermentation used for microbial production of chymosin. The fermentation mixture is filtered to provide a filtrate containing the chymosin. The filtrate is first prepared by either adjusting it to a low pH, such as about 2 and/or adjusting the salt concentration of the filtrate to at least about 2M. The aqueous filtrate mixture is contacted with a phenyl-sepharose resin, which selectively binds the chymosin from the filtrate. The filtrate remaining after the contact with the phenyl-sepharose resin is discarded, and the phenyl-sepharose resin column is eluted with water or dilute salt solution to obtain substantially pure chymosin in a single elution step.

US Patent:

51399431, Aug 18, 1992

Filed:

Jun 13, 1990

Appl. No.:

7/537464

Inventors:

Henry G. Heinsohn - Pacifica CA
Jeffrey D. Lorch - San Mateo CA
Kirk J. Hayenga - San Mateo CA
Raymond E. Arnold - San Francisco CA

Assignee:

Genencor International, Inc. - South San Francisco CA

International Classification:

C12N 964

US Classification:

435226

Abstract:

Disclosed are methods for the recovery of microbially produced chymosin. In particular, disclosed are methods for the recovery of chymosin from the fermentation beer arising from culturing microorganisms which have been engineered so as to produce chymosin. Also disclosed are methods for the selective recovery and subsequent purification of microbially produced chymosin.

US Patent:

20200299745, Sep 24, 2020

Filed:

Apr 6, 2020

Appl. No.:

16/841307

Inventors:

- South San Francisco CA, US
Kirk Hayenga - South San Francisco CA, US
Henry Heinsohn - South San Francisco CA, US

International Classification:

C12P 21/00
C12P 1/04
C12P 21/02
C07K 14/535
C07K 16/00

Abstract:

This invention provides for an improved method of stabilizing freeze dried bacterial extracts with a carbohydrate lyoprotectant such that the extracts can be for use in cell free protein synthesis. Also provided herein are formulations for stable, freeze dried bacterial extracts that when stored at room temperature retain at least about 70% protein synthesis activity compared to undried frozen bacterial extracts.

US Patent:

20180002732, Jan 4, 2018

Filed:

Jan 8, 2016

Appl. No.:

15/542396

Inventors:

- South San Francisco CA, US
Kirk Hayenga - South San Francisco CA, US
Henry Heinsohn - South San Francisco CA, US

International Classification:

C12P 21/00
C07K 16/00
C07K 14/535

Abstract:

This invention provides for an improved method of stabilizing freeze dried bacterial extracts with a carbohydrate lyoprotectant such that the extracts can be for use in cell free protein synthesis. Also provided herein are formulations for stable, freeze dried bacterial extracts that when stored at room temperature retain at least about 70% protein synthesis activity compared to undried frozen bacterial extracts.