Grigoriy S Tchaga

US Patent:

7176298, Feb 13, 2007

Filed:

May 15, 2001

Appl. No.:

09/858332

Inventors:

Grigoriy S. Tchaga - Newark CA, US
George G. Jokhadze - Mountain View CA, US

Assignee:

Clontech Laboratories, Inc. - Mountain View CA

International Classification:

C07H 21/02
C07H 21/04
C12N 15/63
C12N 1/20
C12N 15/00

US Classification:

536 234, 536 231, 4353201, 4352523, 43525233, 435325, 435348

Abstract:

The present invention provides metal ion affinity peptides, fusion proteins comprising metal ion affinity peptides, and polynucleotides encoding the fusion proteins. A feature of the subject invention is that the metal ion affinity peptide has a formula selected from the group consisting of: formula 1: (His-X-X)-(His-X-X-X)-(His-X), wherein each of Xand Xis independently an amino acid with an aliphatic or an amide side chain, each of X, X, Xis independently an amino acid with a basic side chain (except His) or an acidic side chain, each Xis an amino acid with an aliphatic or an amide side chain, n1 and n2 are each independently 1–3, and n3 is 1–5; formula 2: (His-Asn), where n=3 to 10; and formula 3: (His-X-X), wherein each of Xand Xis an amino acid having an acidic side chain, and n=3 to 10. The invention further provides recombinant vectors comprising subject polynucleotides, and host cells comprising the recombinant vectors. The invention further provides methods and kits for purifying a fusion protein comprising a metal ion affinity peptide.

US Patent:

7294614, Nov 13, 2007

Filed:

Oct 11, 2005

Appl. No.:

11/249151

Inventors:

Grigoriy Simeonov Tchaga - Newark CA, US
Rajinder K. Bhatia - Mountain View CA, US

Assignee:

Clontech Laboratories, Inc. - Mountain View CA

International Classification:

C07H 13/00
A23J 7/00

US Classification:

514 2, 530412, 530416, 536117, 536112, 524 39

Abstract:

Phosphorylated protein (i. e. , phosphoprotein) affinity resins and methods for making and using the same are provided. The subject resins include a substrate bonded to aspartate-based tetradentate ligand/metal ion complexes, where the tetradentate ligand/metal ion complexes have high specificity for phosphorylated amino acids. The subject resins find use in a variety of different applications, including phosphoprotein enrichment applications. Also provided are kits and systems that include the subject resins.

US Patent:

7354721, Apr 8, 2008

Filed:

Sep 21, 2001

Appl. No.:

09/960716

Inventors:

Grigoriy S. Tchaga - Newark CA, US

Assignee:

Clontech Laboratories, Inc. - Mountain View CA

International Classification:

G01N 33/53

US Classification:

435 71

Abstract:

Methods of determining whether a sample includes one or more analytes, particularly proteinaceous analytes, of interest are provided. In the subject methods, an array of binding agents, where each binding agent includes an epitope binding domain of an antibody, is contacted with the sample. In many embodiments, contact occurs in the presence of a metal ion chelating polysaccharide, e. g. , a pectin. Following contact, the presence of binding complexes on the array surface are detected and the resultant data is employed to determine whether the sample includes the one or more analytes of interest. Also provided are kits, systems and other compositions of matter for practicing the subject methods. The subject methods and compositions find use in a variety of applications, including proteomic applications such as protein expression analysis, e. g. , differential protein expression profiling.

US Patent:

7723125, May 25, 2010

Filed:

Sep 5, 2007

Appl. No.:

11/850474

Inventors:

Grigoriy S. Tchaga - Newark CA, US

Assignee:

Clontech Laboratories Inc. - Mountain View CA

International Classification:

G01N 33/543

US Classification:

436518

Abstract:

Methods of determining whether a sample includes one or more analytes, particularly proteinaceous analytes, of interest are provided. In the subject methods, an array of binding agents, where each binding agent includes an epitope binding domain of an antibody, is contacted with the sample. In many embodiments, contact occurs in the presence of a metal ion chelating polysaccharide, e. g. , a pectin. Following contact, the presence of binding complexes on the array surface are detected and the resultant data is employed to determine whether the sample includes the one or more analytes of interest. Also provided are kits, systems and other compositions of matter for practicing the subject methods. The subject methods and compositions find use in a variety of applications, including proteomic applications such as protein expression analysis, e. g. , differential protein expression profiling.

US Patent:

20010026919, Oct 4, 2001

Filed:

Dec 28, 2000

Appl. No.:

09/752293

Inventors:

Alex Chenchik - Palo Alto CA, US
Grigoriy Tchaga - Newark CA, US
Peter Simonenko - Mountain View CA, US

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

Hybridization assays, as well as kits, primers and arrays for use in practicing the same, are provided. In the subject assays, a population of tagged target nucleic acids generated from a population of tagged gene specific primers is contacted with an array of tag complements under hybridization conditions and the presence of any resultant hybridized tag target nucleic acid-tag complement structures is detected. The subject arrays find use in a number of different applications, e.g. differential gene expression analysis.

US Patent:

20010031468, Oct 18, 2001

Filed:

Dec 28, 2000

Appl. No.:

09/752292

Inventors:

Alex Chenchik - Palo Alto CA, US
Grigoriy Tchaga - Newark CA, US
Peter Simonenko - Mountain View CA, US

International Classification:

C12Q001/68
G01N033/53
G01N033/537
G01N033/543

US Classification:

435/006000, 435/007920

Abstract:

Analyte detection assays, as well as kits, primers and universal arrays for use in practicing the same, are provided. In many embodiments of the subject assays, a population of tagged affinity ligands is first contacted with a sample being assayed under conditions sufficient to produce binding complexes of tagged affinity ligand/analyte complexes between affinity ligands and their corresponding target analytes present in the sample. The resultant composition is then contacted with a universal array of tag complements under hybridization conditions and the presence of any resultant hybridized or surface bound tagged affinity ligand/analyte-tag complement structures is detected. The subject methods find use in a number of different applications, and are particularly suited for use in proteomics.

US Patent:

20030023037, Jan 30, 2003

Filed:

Jun 20, 2002

Appl. No.:

10/176955

Inventors:

Grigoriy Tchaga - Newark CA, US

International Classification:

C07K009/00
C07K014/00

US Classification:

530/350000, 530/395000

Abstract:

Water-soluble metal ion affinity compounds and methods for using the same are provided. The subject compounds include an aspartate based metal chelating ligand bonded to a water-soluble polymeric substrate, where the ligand is complexed with a metal ion. In certain embodiments, the subject compounds further include a member of a signal producing system, e.g., a directly or an indirectly detectable label moiety. Also provided are water-insoluble supports having the subject compounds present on, e.g., immobilized on, at least one surface thereof. The subject compounds find use in a variety of different applications, including analyte detection and analyte purification applications.

US Patent:

20040180415, Sep 16, 2004

Filed:

Jan 21, 2004

Appl. No.:

10/762588

Inventors:

Grigoriy Tchaga - Newark CA, US
George Jokhadze - Mountain View CA, US

International Classification:

C12N009/00

US Classification:

435/183000, 530/412000

Abstract:

The present invention provides methods of purifying proteins that include a metal ion affinity peptide. The methods generally involve contacting a fusion protein that includes a metal ion affinity peptide with at least two different metal ion chelating resins. In certain representative embodiments, the methods include contacting a fusion protein with a first metal ion chelate resin having a first immobilized metal ion; eluting any bound protein from the first metal ion chelate resin, to produce an eluate; contacting the eluate with a second metal ion chelate resin having a second immobilized metal ion; and eluting any bound protein from the second metal ion chelate resin. Also provided are kits for use in practicing the subject methods. The subject methods find use in a variety of protein purification applications.