Gregory R Milman

US Patent:

52567680, Oct 26, 1993

Filed:

Sep 5, 1990

Appl. No.:

7/578480

Inventors:

Gregory Milman - Potomac MD

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

A61K 39245
C07K 1504
C07K 1300

US Classification:

530350

Abstract:

Bacterially synthesized polypeptides that are recognized by antibodies produced in EBV-infected humans are disclosed. Plasmids containing DNA sequences encoding portions of the EBNA and EBEA-D antigens are also disclosed, as are methods for expressing these DNA sequences in bacteria. The DNA sequence encoding the EBNA-1-related fusion polypeptide is found in a Bam H1 K-restriction fragment, while that encoding the EBEA-D-related fusion polypeptide is found in a Bam H1 M-restriction fragment.

US Patent:

53745205, Dec 20, 1994

Filed:

Jul 15, 1993

Appl. No.:

8/091422

Inventors:

Gregory Milman - Baltimore MD

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

C12Q 100
G01N 3353

US Classification:

435 5

Abstract:

Assay methods and kits for detecting and quantifying anti-Epstein-Barr Virus ("EBV") antibodies in a sample. The methods comprise contacting samples with a free or immobilized synthetic antigenic EBV polypeptide that is recognized by human antibodies and detecting or quantifying immunocomplex formation. The polypeptides described are the carboxy-terminated one-third of the EBV Nuclear Antigen encoded by the EBV Bam H1 K-fragment (plasmid pNAK28), a 46-55 kDa EBV Early Antigen encoded by the 4340 base pair Bcl 1 to Bam H1 restriction fragment within the EBV Bam H1 M-fragment (plasmid pEAD), and an EBV viral capsid antigen. Bacterially synthesized fusion proteins are recognized by human anti-EBV antibodies. The assay method is diagnostic for infectious mononucleosis, chronic mononucleosis, Burkett's lymphoma and monopharyngeal carcinoma.