Gianni C Ferreri

US Patent:

20110003701, Jan 6, 2011

Filed:

Feb 23, 2009

Appl. No.:

12/380139

Inventors:

Gianni Calogero Ferreri - Northford CT, US
Jan F. Simons - San Francisco CA, US
Michael Todd Ronan - New York NY, US
Michael Egholm - Madison CT, US
Brian C. Godwin - North Haven CT, US
David Roderick Riches - Hamden CT, US
Stephen Kyle Hutchison - Branford CT, US
Michael S. Braverman - New Haven CT, US
Melinda D. Palmer - Hamden CT, US
Jeffrey Jeddeloh - Verona WI, US
Jacob Kitzman - Seattle WA, US
Thomas J. Albert - Verona WI, US

International Classification:

C40B 30/04
C07H 21/00
C12P 19/34
C12Q 1/68
C07H 21/04

US Classification:

506 9, 536 231, 435 912, 435 6

Abstract:

An embodiment of an adaptor element for efficient target processing is described that comprises a semi-complementary double stranded nucleic acid adaptor comprising a non-complementary region and a complementary region, where the non-complementary region comprises a first amplification primer site and a second amplification primer site and the complementary region comprises a sequencing primer site and one or more inosine species.

US Patent:

20140073511, Mar 13, 2014

Filed:

Aug 19, 2013

Appl. No.:

13/970167

Inventors:

Gianni Calogero Ferreri - Northford CT, US
Sagnik Basuray - Columbia MO, US
Arthika Bappal - Bridgeport CT, US
Suresh Gopalkrishna Shenoy - Branford CT, US
Xavier Victor Gomes - Wallingford CT, US

International Classification:

C12Q 1/68
H01L 27/105

US Classification:

506 2, 506 16, 257253

Abstract:

An embodiment of a method for sequencing a species of nucleic acid template using pH inert reference sensors is described that comprises the steps of: introducing a nucleotide species to an array of wells where a plurality of the wells comprise a species of nucleic acid template and a plurality of the wells comprise a plurality of functional groups with a high pH buffering characteristic, and in at least a first well a polymerase species incorporates the nucleotide species into a plurality of strands complementary to the species of nucleic acid template disposed in the first well and results in a release of a plurality of hydrogen ions; detecting a signal in the first well that is responsive to the hydrogen ions and one or more noise sources; detecting a signal in a second well comprising the functional groups with the high pH buffering characteristic that is responsive to the one or more noise sources; and subtracting the second well signal from the first well signal to generate a corrected signal associated with the detected hydrogen ions.

US Patent:

20090233291, Sep 17, 2009

Filed:

Jan 28, 2009

Appl. No.:

12/322119

Inventors:

Zhoutao Chen - Carlsbad CA, US
Brian Christopher Godwin - North Haven CT, US
Gianni Calogero Ferreri - Northford CT, US
David Roderick Riches - Hamden CT, US

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

An embodiment of a method for obtaining a DNA construct comprising two end regions of a target nucleic acid in an in vitro reaction is described that comprises the steps of: fragmenting a large nucleic acid molecule to produce a target nucleic acid molecule; ligating a recombination adaptor element to each end of the target nucleic acid molecule to produce an adapted target nucleic acid molecule; exposing the adapted target nucleic acid to a site specific recombinase to produce a circular nucleic acid product and a linear nucleic acid product from the adapted target nucleic acid, wherein the circular nucleic acid product comprises the target nucleic acid molecule; and fragmenting the circular nucleic acid product to produce a template nucleic acid molecule comprising a sequence region from each end of the target nucleic acid molecule.

US Patent:

20220223230, Jul 14, 2022

Filed:

Jan 26, 2022

Appl. No.:

17/584466

Inventors:

- Tucson AZ, US
David Chafin - Tucson AZ, US
Bohuslav Dvorak - Tucson AZ, US
Gianni Ferreri - Tucson AZ, US

International Classification:

G16B 40/20
G16B 25/10
G16H 10/40

Abstract:

The present disclosure relates to automated systems and methods for quantitatively determining an unmasking status of a biological specimen subjected to an unmasking process (e.g. an antigen retrieval process and/or a target retrieval process) using a trained unmasking status estimation engine. In some embodiments, the trained unmasking status estimation engine comprises a machine learning algorithm based on a projection onto latent structure regression model. In some embodiments, the trained unmasking status estimation engine includes a neural network.

US Patent:

20170247734, Aug 31, 2017

Filed:

Mar 8, 2017

Appl. No.:

15/453733

Inventors:

- Branford CT, US
Priya SHANBHAG - Rocky Hill CT, US
Craig Elder MEALMAKER - Jersey City NJ, US
Gianni Calogero FERRERI - Northford CT, US
Melinda PALMER - Hamden CT, US
Shally Hsueh-Wen WANG - Guilford CT, US

International Classification:

C12P 19/34
C12Q 1/68

Abstract:

An embodiment of a method for generating a population of amplified concatamer products is described that comprises amplifying a template nucleic acid molecule using a first nucleic acid primer immobilized on a bead substrate and a second nucleic acid primer in solution to generate a population of substantially identical copies of the template nucleic acid molecule immobilized on the bead substrate; and amplifying the population of substantially identical copies of the template nucleic acid molecule using a concatamer primer that comprises a first region complementary to an end region of the population of substantially identical copies of the template nucleic acid molecule and a second region to generate a population of immobilized concatamer products of the substantially identical copies of the template nucleic acid molecule.

US Patent:

20150056662, Feb 26, 2015

Filed:

Aug 22, 2014

Appl. No.:

14/466063

Inventors:

- Branford CT, US
Priya Shanbhag - Rocky Hill CT, US
Craig Elder Mealmaker - Jersey City NJ, US
Gianni Calogero Ferreri - Northford CT, US
Melinda Palmer - Hamden CT, US
Shally Hsueh-Wen Wang - Guilford CT, US

International Classification:

C12P 19/34

US Classification:

435 912

Abstract:

An embodiment of a method for generating a population of amplified concatamer products is described that comprises amplifying a template nucleic acid molecule using a first nucleic acid primer immobilized on a bead substrate and a second nucleic acid primer in solution to generate a population of substantially identical copies of the template nucleic acid molecule immobilized on the bead substrate; and amplifying the population of substantially identical copies of the template nucleic acid molecule using a concatamer primer that comprises a first region complementary to an end region of the population of substantially identical copies of the template nucleic acid molecule and a second region to generate a population of immobilized concatamer products of the substantially identical copies of the template nucleic acid molecule.