Gerald B Borglum

US Patent:

42129435, Jul 15, 1980

Filed:

Mar 27, 1978

Appl. No.:

5/890500

Inventors:

Gerald B. Borglum - Elkhart IN

Assignee:

Miles Laboratories, Inc. - Elkhart IN

International Classification:

C12K 100
C12K 108

US Classification:

435180

Abstract:

A bacterial cell aggregate having increased particle hardness is produced by contacting a mass of bacterial cells with a cross-linking reaction product of (1) glutaraldehyde, cyanuric halide or combinations thereof and (2) a specific cationic polymer obtained by polymerization of an epihalohydrin and an alkylene polyamine, recovering the resulting aggregate and drying the aggregate.

US Patent:

43473202, Aug 31, 1982

Filed:

Nov 24, 1980

Appl. No.:

6/209411

Inventors:

Gerald B. Borglum - Elkhart IN

Assignee:

Miles Laboratories, Inc. - Elkhart IN

International Classification:

C12P 748
C12P 706
C12N 1110

US Classification:

435144

Abstract:

Microorganisms are immobilized by mixing the microorganisms with an aqueous solution of kappacarrageenan and gelling the resultant mixture by the addition of an epihalohydrin: alkylene polyamine polymer having a mole ratio of from about 0. 60:1 to 2. 7:1 and a molecular weight of from 4,000 to 50,000 or by the addition of polyethyleneimine having a molecular weight of 300 to 50,000. Preferred microorganisms are Aspergillus niger and Saccharomyces cerevisiae which are respectively used to produce citric acid and ethanol.