Geoffrey A Dafforn

US Patent:

8143001, Mar 27, 2012

Filed:

Nov 30, 2007

Appl. No.:

11/948784

Inventors:

Nurith Kurn - Palo Alto CA, US
Geoffrey A. Dafforn - Los Altos CA, US

Assignee:

NuGEN Technologies, Inc. - San Carlos CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04

US Classification:

435 61, 435 62, 435 911, 536 231, 536 243, 536 2433

Abstract:

The invention relates to methods for analysis of nucleic acid methylation status, and fragmentation and/or labeling and/or immobilization of nucleic acids. More particularly, the invention relates to methods for fragmentation and/or labeling and/or immobilization of nucleic acids comprising labeling and/or cleavage and/or immobilization at abasic sites.

US Patent:

20040005614, Jan 8, 2004

Filed:

May 19, 2003

Appl. No.:

10/441663

Inventors:

Nurith Kurn - Palo Alto CA, US
Geoffrey Dafforn - Los Altos CA, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

The invention relates to methods for fragmentation and/or labeling and/or immobilization of nucleic acids. More particularly, the invention relates to methods for fragmentation and/or labeling and/or immobilization of nucleic acids comprising labeling and/or cleavage and/or immobilization at abasic sites.

US Patent:

20040023271, Feb 5, 2004

Filed:

Mar 31, 2003

Appl. No.:

10/404895

Inventors:

Nurith Kurn - Palo Alto CA, US
Geoffrey Dafforn - Los Altos CA, US

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

The present invention provides novel methods of indirect analyte dectection and quantification through amplification of oligonucleotide template attached to binding partners for analytes by nucleic acid amplification utilizing isothermal, single primer linear nucleic acid amplification methods. Methods for binding of binding partner that is attached to an oligonucleotide template to analyte, then amplifying at least a portion of the oligonucleotide template using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. Methods for detecting and quantifying amplification products are also provided. The invention further provides compositions and kits for practicing said methods.

US Patent:

20050208538, Sep 22, 2005

Filed:

Dec 29, 2004

Appl. No.:

11/026280

Inventors:

Nurith Kurn - Palo Alto CA, US
Geoffrey Dafforn - Los Altos CA, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435006000, 435091200

Abstract:

The invention relates to methods for analysis of nucleic acid methylation status, and fragmentation and/or labeling and/or immobilization of nucleic acids. More particularly, the invention relates to methods for fragmentation and/or labeling and/or immobilization of nucleic acids comprising labeling and/or cleavage and/or immobilization at abasic sites.

US Patent:

49817868, Jan 1, 1991

Filed:

Sep 4, 1987

Appl. No.:

7/094176

Inventors:

Geoffrey A. Dafforn - Los Altos CA
Nurith Kurn - Palo Alto CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA

Assignee:

Syntex (U.S.A.) Inc. - Palo Alto CA

International Classification:

G01N 33535
G01N 2101

US Classification:

435 7

Abstract:

A device is disclosed for conducting an assay. The device comprises a housing, means enclosed in the housing for capturing a first member of a specific binding pair (sbp) in a zone and for allowing liquid to be transported by capillary action away from the zone, first means in the housing for introducing the sample into the device, and second means in the housing other than the first means for introducing a liquid reagent other than the sample into the device. The device of the invention finds use in assay methods for the determination of an analyte in a sample suspected of containing the analyte. Kits for conducting an assay are also disclosed.