Frederic Kendirgi

US Patent:

20080305142, Dec 11, 2008

Filed:

Dec 12, 2005

Appl. No.:

11/792800

Inventors:

Yin Chen - Pearland TX, US
Frederic Kendirgi - Houston TX, US
Malcolm Skolnick - Houston TX, US

Assignee:

CYTOGENIX, INC. - HOUSTON TX

International Classification:

A61F 2/04
C12P 19/34
A01N 43/00
A61K 39/00
A61P 31/12
A61P 31/10
A61K 31/7052
A61K 9/12

US Classification:

424423, 435 915, 504209, 514 44, 424 45, 4241841, 4352861

Abstract:

The invention provides an improved cell free amplification method capable of producing large quantities of therapeutic-quality nucleic acids and methods of using the synthesized nucleic acid in research, therapeutic and other applications—The methods combine several different state-of-the-art procedures and coordinate their applications to affordably synthesize nucleic acids for therapeutic purposes. It combines in vitro rolling circle amplification, high fidelity polymerases, high affinity primers, and streamlined template specifically designed for particular applications. For expression purposes, the templates contain an expression cassette including a eukaryotic promoter, the coding sequence for the gene of interest, and a eukaryotic termination sequence. Following amplification, concatamers are subsequently processed according to their intended use and may include: restriction enzyme digestion for the production of short expression cassettes (SECs); ligation steps to circularize the SEC (CNAs); and/or supercoiling steps to produce sCNAs. The final product contains nearly non-detectable levels of bacterial endotoxin.

US Patent:

20210321621, Oct 21, 2021

Filed:

Jun 5, 2019

Appl. No.:

15/734484

Inventors:

- Hong Kong, HK
Frederic Kendirgi - Woodland CA, US

International Classification:

A01N 63/20
C12Q 1/689
C12N 9/88
C07K 14/195
A01N 63/28
A01N 63/22
A01N 63/25
C05F 11/08
C05G 1/00
C05G 3/60
C05G 5/20
C05G 5/40

Abstract:

Methods for selecting a microbe for co-formulation with a carrier are provided. In some examples, the methods include identifying a microbe that comprises one or more dipicolinic acid (DPA) synthase genes, a microbe that expresses one or more DPA synthase proteins, and/or a microbe that produces DPA; and selecting the microbe for co-formulation with a carrier. The methods optionally include co-formulating the selected microbe with the carrier. In some examples, the methods include detecting one or more DPA synthase genes or one or more DpaA and/or DpaB proteins in a microbe. In other examples, the methods include detecting DPA in a microbe or medium containing a microbe, for example, utilizing a fluorescence assay. Microbial compositions including one or more microbes that comprise one or more DPA synthase genes, express one or more DPA synthase proteins and/or produce DPA are also provided.