Erich B Jaeger

US Patent:

20200216895, Jul 9, 2020

Filed:

Jul 31, 2018

Appl. No.:

16/626115

Inventors:

- San Diego CA, US
Victoria Popic - San Jose CA, US
Erich B. Jaeger - San Francisco CA, US
Mostafa Ronaghi - Atherton CA, US

International Classification:

C12Q 1/6874
C12Q 1/6813

Abstract:

Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

US Patent:

20190249171, Aug 15, 2019

Filed:

Feb 11, 2019

Appl. No.:

16/272870

Inventors:

- San Diego CA, US
Tarun Kumar Khurana - Fremont CA, US
Victoria Popic - San Francisco CA, US
Erich B. Jaeger - San Francisco CA, US
Mostafa Ronaghi - San Francisco CA, US

International Classification:

C12N 15/10
C12Q 1/6806
B01L 3/00
B01L 7/00

Abstract:

Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.