Skills:
APPLIED BIOLOGICAL SKILLS: (1) PCR, reverse transcription, enzymatic reactions, cosmid library manipulation, sequencing and sequence analysis (Beckman Coulter CEQ8000), electrophoresis; (2) nucleic acid preparation from blood, semen, murine heart tissue, cell cultures, bacteria; (3) microarray design, production, sample preparation, hybridization, imaging, Southern blotting, transcription factor/DNA binding experiments in solution and on microarrays (experience with Amersham Typhoon 9410 fluorescence detection system and Axon Genepix 3000 microarray imager); (4) construction of bacterial plasmid and bacteriophage vectors, transfection of streptomycetes, transformation of Escherichia coli and streptomycetes, culture of bacteria, yeast, human cell lines, and plant tissue callus; (5) mouse handling, anesthesia, surgery, necropsy, echocardiography; (6) compounding, HPLC, and spectroscopic analysis of chow and aqueous solutions containing drugs; (7) SensQ and SensQ Pioneer assay development, micro-ring resonators, ELISA. ANALYSIS PACKAGES USED: (1) SPR packages: Qdat, Biacore, SensQ and SensQ Pioneer software; (2) Other packages: Beckman Coulter CEQ2000 and CEQ8000 sequencing software, Phred/Phrap/Consed/PolyPhred, DNAStar Lasergene, Genecodes Sequencher, Primer3, CLUSTAL, the NCBI and PDB websites, BLAST, BioEdit, ChemDraw, CloneManager.