Eddie E Bautista

US Patent:

20080124762, May 29, 2008

Filed:

Jul 31, 2007

Appl. No.:

11/831882

Inventors:

Masahisa Handa - Berkeley CA, US
Arnold Horwitz - San Leandro CA, US
Eddie Bautista - San Francisco CA, US
Robyn Cotter - Oakland CA, US

Assignee:

XOMA TECHNOLOGY LTD. - Berkeley CA

International Classification:

C12P 21/04
C12N 15/00
C12N 5/06

US Classification:

435 691, 4353201, 435325, 435366, 435369

Abstract:

Recombinant expression vectors are provided comprising a 3′ UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.

US Patent:

20080145894, Jun 19, 2008

Filed:

Jul 31, 2007

Appl. No.:

11/831691

Inventors:

Masahisa Handa - Berkeley CA, US
Arnold Horwitz - San Leandro CA, US
Eddie Bautista - San Francisco CA, US
Robyn Cotter - Oakland CA, US

Assignee:

XOMA TECHNOLOGY LTD. - Berkeley CA

International Classification:

C12P 21/04

US Classification:

435 691

Abstract:

Recombinant expression vectors are provided comprising a 3′UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.

US Patent:

20060121604, Jun 8, 2006

Filed:

Dec 5, 2005

Appl. No.:

11/295006

Inventors:

Masahisa Handa - Berkeley CA, US
Arnold Horwitz - San Leandro CA, US
Eddie Bautista - San Francisco CA, US
Robyn Cotter - Oakland CA, US

Assignee:

XOMA Technology Ltd. - Hamilton

International Classification:

C12Q 1/68
C12N 15/63
C12N 15/74
C12N 15/00
C12N 15/70
C12N 15/09

US Classification:

435320100, 435006000

Abstract:

Recombinant expression vectors are provided comprising a 3′ UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.