Dmitriy Y Gremyachinskiy

US Patent:

20110003343, Jan 6, 2011

Filed:

Mar 26, 2010

Appl. No.:

12/748355

Inventors:

Theo Nikiforov - Carlsbad CA, US
Daniel Mazur - San Diego CA, US
Xinzhan Peng - Carlsbad CA, US
Yuri Belosludtsev - The Woodlands TX, US
Howard Reese - Poway CA, US
Dmitriy Gremyachinskiy - Eugene OR, US
Roman Rozhkov - Eugene OR, US
John M. Mauro - Eugene OR, US
Joseph Beechem - Eugene OR, US
Eric Tulsky - Eugene OR, US
Imad Naasani - Manchester, GB
Kari Haley - Eugene OR, US
Joseph A. Treadway - Eugene OR, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 19/34
C12N 9/10

US Classification:

435 915, 435193

Abstract:

Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

US Patent:

20130211006, Aug 15, 2013

Filed:

Feb 8, 2013

Appl. No.:

13/763066

Inventors:

LIFE TECHNOLOGIES CORPORATION - , US
Alan Blanchard - Middleton MA, US
Luisa Andruzzi - Beverly MA, US
Shaheer Khan - Foster City CA, US
Dmitriy Gremyachinskiy - Eugene OR, US
Alfred Lui - Carlsbad CA, US
Craig Stolarczyk - San Mateo CA, US
Tanya Sokolsky - Cambridge MA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C08F 8/40

US Classification:

525 542

Abstract:

A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

US Patent:

20130211119, Aug 15, 2013

Filed:

Feb 8, 2013

Appl. No.:

13/762489

Inventors:

LIFE TECHNOLOGIES CORPORATION - , US
Geir Fonnum - Fjellhamar, NO
Bruce Branchaud - Eugene OR, US
Steven Menchen - Fremont CA, US
Dmitriy Gremyachinskiy - Eugene OR, US
Hee Chol Kang - Eugene OR, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C07F 7/18

US Classification:

556419

Abstract:

A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

US Patent:

20140073756, Mar 13, 2014

Filed:

Nov 15, 2013

Appl. No.:

14/081224

Inventors:

- Oslo, NO
Bruce BRANCHAUD - Eugene OR, US
Steven M. MENCHEN - Fremont CA, US
Dmitriy GREMYACHINSKIY - San Francisco CA, US
Hee Chol KANG - Eugene OR, US

Assignee:

LIFE TECHNOLOGIES AS - Oslo
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C07F 7/18
C08F 236/20

US Classification:

526279, 556419

Abstract:

A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

US Patent:

20140080966, Mar 20, 2014

Filed:

Nov 15, 2013

Appl. No.:

14/081263

Inventors:

- Oslo, NO
Bruce BRANCHAUD - Eugene OR, US
Steven M. MENCHEN - Fremont CA, US
Dmitriy GREMYACHINSKIY - San Francisco CA, US
Hee Chol KANG - Eugene OR, US

Assignee:

LIFE TECHNOLOGIES AS - Oslo
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C07F 7/10
C08K 3/20
C08F 236/20

US Classification:

524547, 556419, 526279

Abstract:

A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

US Patent:

20210088511, Mar 25, 2021

Filed:

Sep 30, 2020

Appl. No.:

17/039245

Inventors:

- Indianapolis IN, US
Dmitriy GREMYACHINSKIY - Sunnyvale CA, US
Dieter HEINDL - Muenchen, DE
Hannes KUCHELMEISTER - Muenchen, DE
Michael SCHRAEML - Penzberg, DE
Andrew TRANS - Mountain View CA, US

Assignee:

Roche Diagnostics Operations, Inc. - Indianapolis IN

International Classification:

G01N 33/543
G01N 33/487

Abstract:

Provided are nanopore-based methods, compositions, and systems for assessing analyte-ligand interactions and analyte concentration in a fluid solution. The compositions include an analyte detection complex that is associated with a nanopore to form a nanopore assembly, the analyte detection complex including an analyte ligand. As a first voltage is applied across the nanopore assembly, the analyte ligand is presented to an analyte in the solution. As a second voltage that is opposite in polarity to the first voltage is applied across the nanopore assembly, the analyte binds to the analyte. By comparing the total number of analyte-ligand binding pairs to a control binding count, the concentration of the analyte can be determined. In other examples, further increasing the second voltage can result in dissociation of the analyte-ligand pair, from which a dissociation voltage—and hence a dissociation constant—can be determined.

US Patent:

20200354780, Nov 12, 2020

Filed:

Jul 24, 2020

Appl. No.:

16/938192

Inventors:

- Carlsbad CA, US
Wolfgang HINZ - Killingworth CT, US
Ronald CICERO - Menlo Park CA, US
Christina INMAN - San Mateo CA, US
Paul KENNEY - Sunnyvale CA, US
Alexander MASTROIANNI - Alameda CA, US
Roman ROZHKOV - Carlsbad CA, US
Yufang WANG - San Carlos CA, US
Jeremy GRAY - Larkspur CA, US
Marc GLAZER - San Jose CA, US
Dmitriy GREMYACHINSKIY - San Francisco CA, US

International Classification:

C12Q 1/6837
C12Q 1/6869
C12Q 1/6834
C12Q 1/6806

Abstract:

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

US Patent:

20200354789, Nov 12, 2020

Filed:

Jul 24, 2020

Appl. No.:

16/938222

Inventors:

- Carlsbad CA, US
Alan BLANCHARD - Topsfield MA, US
Luisa ANDRUZZI - Beverly MA, US
Shaheer KHAN - Foster City CA, US
Dmitriy GREMYACHINSKIY - San Francisco CA, US
Alfred LUI - Sunnyvale CA, US
Craig STOLARCZYK - San Mateo CA, US
Tanya SOKOLSKY - Cambridge MA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/6876
C12Q 1/6834
C08F 8/40

Abstract:

A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.