Diether J Recktenwald

US Patent:

7507588, Mar 24, 2009

Filed:

Apr 14, 2006

Appl. No.:

11/404348

Inventors:

Majid Mehrpouyan - Gilroy CA, US
Diether J. Recktenwald - San Jose CA, US
Rudolf Varro - Mountain View CA, US

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

G01N 33/543

US Classification:

436518, 435 4, 435 6, 435 71, 435 72, 4352872, 436517, 436523, 436524, 436525, 436526, 436527, 436528, 436529, 436530, 436531, 436546, 436 44, 436 46, 436164, 436172

Abstract:

Arrays of microparticle populations, each population labeled with a single fluorescent dye, are provided for use in multiplex assays. The populations form a virtual multidimensional array wherein each microparticle is identified by fluorescence intensity in two different fluorescence detection channels. The arrays are useful in a variety of assays, including multiplex, multi-analyte assays for the simultaneous detection of two or more analytes by, for example, flow cytometry, and a labeling reagents in, for example, microscopy. The use of singly-dyed microparticles to form multidimensional arrays greatly simplifies the creation of multiplex assays.

US Patent:

7781227, Aug 24, 2010

Filed:

Mar 4, 2009

Appl. No.:

12/398119

Inventors:

Majid Mehrpouyan - Gilroy CA, US
Diether J. Recktenwald - San Jose CA, US
Rudolf Varro - Mountain View CA, US

Assignee:

Becton, Dickinson and Company - Franklin Lakes NY

International Classification:

G01N 33/543
G01N 33/53

US Classification:

436523, 435 71, 4352872, 435973, 436518, 436524, 436528, 436546, 436 10, 436164, 436172, 422 8208

Abstract:

Arrays of microparticle populations, each population labeled with a single fluorescent dye, are provided for use in multiplex assays. The populations form a virtual multidimensional array wherein each microparticle is identified by fluorescence intensity in two different fluorescence detection channels. The arrays are useful in a variety of assays, including multiplex, multi-analyte assays for the simultaneous detection of two or more analytes by, for example, flow cytometry, and a labeling reagents in, for example, microscopy. The use of singly-dyed microparticles to form multidimensional arrays greatly simplifies the creation of multiplex assays.

US Patent:

48761907, Oct 24, 1989

Filed:

Oct 21, 1987

Appl. No.:

7/111209

Inventors:

Diether J. Recktenwald - Cupertino CA

Assignee:

Becton Dickinson & Company - Franklin Lakes NJ

International Classification:

G01N 2177
G01N 33533

US Classification:

435 7

Abstract:

Peridinin-chlorophyll-protein complexes are provided for use as fluorescent labels and are particularly useful in diagnostic assays employing as a reagent a fluorescent compound conjugated to a member of a specific binding pair, wherein the pair consists of a biochemical ligand and a receptor and the diagnostic assay comprises a step in which the conjugate binds to its complementary binding-pair member.

US Patent:

47452854, May 17, 1988

Filed:

Aug 21, 1986

Appl. No.:

6/898685

Inventors:

Diether J. Recktenwald - Cupertino CA
Chia H. Chen - San Jose CA

Assignee:

Becton Dickinson and Company - Franklin Lakes NJ

International Classification:

G01N 2164

US Classification:

2504581

Abstract:

A method for determining one or more characteristics of particles using multiple fluorescence analysis comprises directing an incident light beam at the particles under analysis. The particles include at least three fluorescent markers each having different emission spectra. The incident light beam causes the excitation of the markers by light at a single wavelength whereby different wavelengths of fluorescence are emitted from the particles. Different fluorescence emissions associated with the particles under analysis are simultaneously detected. This method further includes associating the detected fluorescence with one or more characteristics of the particles. An apparatus is also part of the present invention for carrying out the aforementioned method.

US Patent:

53246292, Jun 28, 1994

Filed:

Apr 27, 1993

Appl. No.:

8/053117

Inventors:

Diether J. Recktenwald - Cupertino CA

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

A01N 102
C12Q 102
C12M 124

US Classification:

435 2

Abstract:

A method for improving recovery of cells from liquid suspension by centrifugation. The method comprises coating the interior of centrifuge containers with a solution comprising an amphipathic compound prior to introduction of the cell suspension and centrifugation. The method is particularly suited for recovery or concentration of rare cells from dilute suspensions, for example when rare cells are isolated from a sample by sorting on a flow cytometer.

US Patent:

52328287, Aug 3, 1993

Filed:

Mar 9, 1992

Appl. No.:

7/848513

Inventors:

Diether J. Recktenwald - Cupertino CA

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

A01N 102

US Classification:

435 2

Abstract:

A method for improving recovery of cells from liquid suspension by centrifuqation. The method comprises coating the interior of centrifuge containers with a solution comprising an amphipathic compound prior to introduction of the cell suspension and centrifugation. The method is suited for recovery or concentration of rare cells from dilute suspensions, for example when rare cells are isolated from a sample by sorting on a flow cytometer.

US Patent:

47048913, Nov 10, 1987

Filed:

Aug 29, 1986

Appl. No.:

6/901860

Inventors:

Diether J. Recktenwald - Cupertino CA
Rickie S. Kerndt - Cupertino CA
Michael R. Loken - Los Altos CA
Chia H. Chen - San Jose CA

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

G06M 1100

US Classification:

73 1R

Abstract:

A method for calibrating an instrument for using that instrument to obtain at least one light-related signal from particles under analysis comprises directing an incident beam of light at calibration particles having one or more known characteristics related to the particles expected to be analyzed. Both a light signal and a noise signal from the calibration particles are detected. A measurement is made of the ratio of the detected light signal to the detected noise signal, and that measurement is reported. The measured ratio is then compared to a predetermined ratio which represents a threshold for minimum instrument performance. This method further includes adjusting, if the predetermined ratio has not been attained, the operation of the instrument, while the calibration particles are within the incident beam of light, until the measured ratio reaches the predetermined ratio whereby the instrument is calibrated for subsequently obtaining the light signal from particles to be analyzed. A kit of reagents for use in calibrating an instrument for performing two-color analysis of particles and particle standards for calibrating fluorescence analysis instruments are also part of the instant invention.

US Patent:

47270209, Feb 23, 1988

Filed:

Feb 25, 1985

Appl. No.:

6/705252

Inventors:

Diether J. Recktenwald - Cupertino CA

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

C12Q 104
C12Q 168
G01N 33554
G01N 33577

US Classification:

435 6

Abstract:

A method for identifying and enumerating cells of a subclass of blood cells in relation to cells of another subclass of blood cells is provided. In the method a first subclass of blood cells is selectively tagged by incubating an aliquot of a blood sample with a first tagging agent. A second subclass of blood cells is selectively tagged by incubating the aliquot with a second tagging agent. The aliquot is then passed, without lysing of any subclasses of blood cells which are not of interest, substantially a cell at a time through an area of optical stimulation for the tagging agents. Light emitted by the tagging agents is detected, the detection being limited by gating to a threshold value related to a predetermined intensity of light by one of the tagging agents. Cells of the subclass are differentiated based on occurrence of emitted light from the tagging agents.

Author:

Diether Recktenwald

ISBN #:

0824798643